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产品描述：人补体7(C7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C7含量。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研场景中探索C7在感染性疾病（如细菌/病毒感染）、自身免疫疾病（如系统性红斑狼疮）或心血管疾病（如动脉粥样硬化）中的调控机制，也可用于评估药物干预或基因修饰对补体系统的影响。实验支持多种生物样本类型，为研究补体通路功能、炎症反应及免疫相关疾病提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit
缩写：C7
Uniprot No.：P10643
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 87-95 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 94-101 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 91-98 | |||||
回收率：
| The recovery of human C7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 99 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.9342.8802.9072.8065002.1022.1952.1492.0482501.4211.5041.4631.3621250.8070.7720.7900.68962.50.4430.4590.4510.35031.20.2690.2590.2640.16315.60.1710.1670.1690.06800.0990.1030.101 | ||||||
货期：3-5 working days
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