首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体成分C6(C6)(C6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C6含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品特别适用于基础医学研究、免疫学实验或疾病模型中C6蛋白表达水平的动态监测，为探究补体系统在感染、自身免疫性疾病或组织损伤等病理过程中的作用提供可靠工具，所有检测结果仅限科学研究使用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AW111623 ELISA Kit; C6 ELISA Kit; CO6_HUMAN ELISA Kit; Complement component 6 ELISA Kit; Complement component C6 ELISA Kit
缩写：C6
Uniprot No.：P13671
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:10000 | Average % | 88 | |||||
| Range % | 81-92 | ||||||
| 1:20000 | Average % | 95 | |||||
| Range % | 91-105 | ||||||
| 1:40000 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
| 1:80000 | Average % | 93 | |||||
| Range % | 86-98 | ||||||
回收率：
| The recovery of human C6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-98 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 40002.0212.1072.0641.940 20001.7451.7841.7651.641 10001.3181.3291.3241.200 5000.9020.8940.8980.774 2500.6510.6680.6600.536 1250.4890.4920.4910.367 62.50.3370.3410.3390.215 00.1220.1250.124 | |||||||
货期：3-5 working days
×
