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产品描述：人补体C1q亚基A(C1QA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的C1QA含量。C1QA 是补体系统经典激活途径起始分子 C1 复合物的亚基。其背景在于补体系统对免疫防御等至关重要。研究机制方面，聚焦于它如何参与补体激活、介导免疫反应、清除病原体及异常细胞，以及在相关疾病如神经系统疾病、自身免疫病中的作用。试剂盒检测范围为9.38 ng/mL-600 ng/mL，适用于基础研究领域中对补体系统功能机制的探索、疾病模型的生物标志物分析以及药物干预效果的体外评价，为免疫学、分子病理学及生物医药研发提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C1qa ELISA Kit; C1QA_HUMAN ELISA Kit; Complement C1q subcomponent subunit A ELISA Kit; Complement component 1 q subcomponent A chain ELISA Kit; Complement component 1 q subcomponent alpha polypeptide ELISA Kit; Complement component C1q A chain ELISA Kit
缩写：C1QA
Uniprot No.：P02745
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：9.38 ng/mL-600 ng/mL
灵敏度：2.34 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C1QA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 90-101 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 93-101 | |||||
回收率：
| The recovery of human C1QA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-97 | ||||
| EDTA plasma (n=4) | 93 | 86-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 6002.7152.6662.6912.594 3001.9861.9941.9901.893 1501.1781.1431.1611.064 750.6620.6520.6570.560 37.50.4130.4020.4080.311 18.750.2870.2740.2810.184 9.380.1690.1630.1660.069 00.0980.0960.097 | ||||||
货期：3-5 working days
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