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产品描述：人血小板受体Gi24(C10orf54)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的C10orf54含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于心血管疾病机制研究、血小板功能体外实验、药物筛选模型建立等科研场景，为探索Gi24在细胞信号传导、病理生理学调控中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B7 H5 ELISA Kit; B7H5 ELISA Kit; C10orf54 ELISA Kit; chromosome 10 open reading frame 54 ELISA Kit; DD1alpha ELISA Kit; GI24 ELISA Kit; GI24_HUMAN ELISA Kit; PD-1H ELISA Kit; PDCD1 homolog ELISA Kit; Platelet receptor Gi24 ELISA Kit; PP2135 ELISA Kit; sisp 1 ELISA Kit; SISP1 ELISA Kit; stress induced secreted protein 1 ELISA Kit; UNQ730/PRO1412 ELISA Kit; V domain Ig suppressor of T cell activation ELISA Kit; V set domain containing immunoregulatory receptor ELISA Kit; V set immunoregulatory receptor ELISA Kit; V type immunoglobulin domain containing suppressor of T cell activation ELISA Kit; VISTA ELISA Kit
缩写：C10orf54
Uniprot No.：Q9H7M9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C10orf54 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 83-89 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 81-89 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human C10orf54 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 98-104 | |||||
| EDTA plasma (n=4) | 99 | 95-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.3662.3052.3362.21351.6421.6581.6501.5272.50.8980.8680.8830.7601.250.4750.4550.4650.3420.6250.3200.3260.3230.2000.3120.2490.2510.2500.1270.1560.1950.1930.1940.07100.1250.1200.123 | |||||||
货期：3-5 working days
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