首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肾胺酶(RNLS/ MAO-C)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C10orf59含量。试剂盒检测范围为0.625 μg/mL-40 μg/mL，该产品适用于心血管疾病机制研究、代谢相关蛋白互作分析，以及通过检测不同处理条件下生物样本中RNLS/MAO - C的动态变化，探索其在氧化应激或能量代谢中的调控作用，为相关疾病模型的分子机制解析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：6530404N21Rik ELISA Kit; AI452315 ELISA Kit; AW060440 ELISA Kit; C10orf59 ELISA Kit; Chromosome 10 open reading frame 59 ELISA Kit; FLJ11218 ELISA Kit; HGNC:25641 ELISA Kit; Hypothetical protein LOC55328 ELISA Kit; MAO C ELISA Kit; MAO-C ELISA Kit; mMAO C ELISA Kit; Monoamine oxidase C ELISA Kit; Monoamine oxidase-C ELISA Kit; Renalase ELISA Kit; Renalase FAD dependent amine oxidase ELISA Kit; RNLS ELISA Kit; RNLS_HUMAN ELISA Kit
缩写：C10orf59
Uniprot No.：Q5VYX0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 μg/mL-40 μg/mL
灵敏度：0.156 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of human RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 82-94 | ||||
| EDTA plasma (n=4) | 97 | 93-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µg/mlOD1OD2AverageCorrected402.5152.4142.4652.321201.7331.6321.6831.539100.9710.9920.9820.83850.5680.5570.5630.4192.50.4020.4140.4080.2641.250.2850.2740.2800.1360.6250.2270.2210.2240.08000.1440.1430.144 | ||||||
货期：3-5 working days
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