首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白D(APOD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOD含量。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于基础科研中对脂代谢机制、神经退行性疾病病理研究以及肿瘤微环境分析的探索。通过检测不同病理模型或干预条件下APOD的动态表达，可为相关疾病的分子机制研究提供可靠数据支持，满足细胞实验、动物模型及临床前研究的多样化需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APO D ELISA Kit; Apo-D ELISA Kit; ApoD ELISA Kit; APOD protein ELISA Kit; APOD_HUMAN ELISA Kit; Apolipoprotein D ELISA Kit; ApolipoproteinD ELISA Kit
缩写：APOD
Uniprot No.：P05090
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:2000 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4000 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8000 | Average % | 85 | ||||
| Range % | 81-90 | |||||
回收率：
| The recovery of human APOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.5022.5682.5352.4222002.2282.2682.2482.1351001.6471.6681.6581.545501.0351.0601.0480.935250.5890.5710.5800.46712.50.3440.3220.3330.2206.250.2110.2130.2120.09900.1110.1150.113 | ||||||
货期：3-5 working days
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