首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白C-IV(APOC4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOC4含量。试剂盒检测范围为6.25 ng/mL-400 ng/mL，科研人员可将其应用于脂代谢异常疾病模型构建、基因调控机制探索或生物样本中APOC4表达水平评估等实验场景，为分子机制研究提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APO C4 ELISA Kit; Apo CIV ELISA Kit; Apo-CIV ELISA Kit; APOC 4 ELISA Kit; ApoC IV ELISA Kit; ApoC-IV ELISA Kit; APOC4 ELISA Kit; APOC4_HUMAN ELISA Kit; Apolipoprotein C IV ELISA Kit; Apolipoprotein C-IV ELISA Kit; Apolipoprotein C4 ELISA Kit
缩写：APOC4
Uniprot No.：P55056
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:1600 | Average % | 97 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average4000.0800.0830.0822000.1170.1190.1181000.1770.1720.175500.2760.2960.286250.5040.4900.49712.50.9230.9310.9276.251.4611.4961.47902.5392.5872.563 | ||||||
货期：3-5 working days
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