首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白C-III(APOC3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOC3含量。APOC3是一种重要的靶点。它在脂质代谢中发挥关键作用，其异常表达与高脂血症等疾病密切相关。研究机制主要集中于探究其调控血脂的具体分子通路，旨在通过干预APOC3来调节血脂水平，为相关心血管疾病的防治提供新策略。试剂盒检测范围为18.75 ng/mL-1200 ng/mL，该产品适用于高脂血症、糖尿病或动脉粥样硬化等代谢性疾病小鼠模型中APOC3的动态监测，也可用于探究肝脏、脂肪组织等器官中APOC3的表达分布及调控机制。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoc3Apolipoprotein C-III ELISA kit; Apo-CIII ELISA kit; ApoC-III ELISA kit; Apolipoprotein C3 ELISA kit
缩写：APOC3
Uniprot No.：P33622
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 ng/mL-1200 ng/mL
灵敏度：9.375 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 88-95 | |||||
| 1:400 | Average % | 83 | ||||
| Range % | 80-87 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of mouse APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-103 | ||||
| EDTA plasma (n=4) | 86 | 82-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 12000.0870.0890.088 6000.1880.1910.190 3000.3820.3780.380 1500.6530.6450.649 751.1311.1461.139 37.51.5161.5271.522 18.751.8191.9211.870 02.3692.2652.317 | ||||||
货期：3-5 working days
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