首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人载脂蛋白C3(apo-C3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOC3含量。APOC3是一种在肝脏和小肠合成的糖蛋白，是富含甘油三酯脂蛋白的主要组分，在血脂代谢中起重要作用。研究表明，APOC3可通过抑制脂蛋白脂酶活性来增加血浆甘油三酯水平，并干扰LDLR和LRP1对TRLs及其残余物的清除，增加甘油三酯和胆固醇水平。研究显示，APOC3可作为降低血脂异常和心血管疾病风险的新兴治疗靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研场景中apo - C3的生物学功能研究，例如心血管疾病模型构建、代谢综合征机制探索或脂代谢异常相关药物开发中的疗效评估，为体外实验提供可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOC3 ELISA Kit; APO C3 ELISA Kit; Apo CIII ELISA Kit; Apo-CIII ELISA Kit; APOC 3 ELISA Kit; ApoC III ELISA Kit; ApoC-III ELISA Kit; APOC3 ELISA Kit; APOC3_HUMAN ELISA Kit; ApoCIII ELISA Kit; Apolipoprotein C III ELISA Kit; Apolipoprotein C-III ELISA Kit; Apolipoprotein C3 ELISA Kit; ApolipoproteinCIII ELISA Kit; MGC150353 ELISA Kit
缩写：APOC3
Uniprot No.：P02656
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 81-94 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 91-105 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 90-105 | |||||
回收率：
| The recovery of human apo-C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-98 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 10000.0800.0850.083 5000.1260.1230.125 2500.2000.2090.205 1250.3630.3700.367 62.50.5870.6120.600 31.250.8040.8230.814 15.61.1121.1641.138 01.9441.8781.911 | ||||||
货期：3-5 working days
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