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产品描述:人载脂蛋白C2(apo-C2)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的APOC2含量。APOC2是重要靶点。它是载脂蛋白C-Ⅱ,在脂质代谢中起关键作用。研究机制表明,APOC2能激活脂蛋白脂肪酶,促进乳糜微粒和极低密度脂蛋白分解代谢。对其深入研究,或为高脂血症等脂质相关疾病治疗提供新方向。试剂盒检测范围为31.2 ng/mL-2000 ng/mL,该产品可广泛应用于基础医学研究中apo-C2表达水平的动态监测,例如探究脂代谢调控机制、评估心血管疾病动物模型干预效果,或结合组织样本分析apo-C2在特定器官中的分布特征。其宽泛的样本兼容性为体外实验与离体研究提供灵活选择,适用于细胞实验、病理模型构建及生物标志物筛查等科研场景,为脂蛋白相关领域研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:APC 2 ELISA Kit; APC2 ELISA Kit; Apo CII ELISA Kit; Apo-CII ELISA Kit; APOC 2 ELISA Kit; ApoC II ELISA Kit; ApoC-II ELISA Kit; APOC2 ELISA Kit; APOC2 protein ELISA Kit; APOC2_HUMAN ELISA Kit; ApoCII ELISA Kit; Apolipoprotein C II ELISA Kit; Apolipoprotein C II precursor ELISA Kit; Apolipoprotein C2 ELISA Kit; ApolipoproteinCII ELISA Kit; MGC75082 ELISA Kit; ProapoC-II ELISA Kit; Proapolipoprotein C-II ELISA Kit
缩写:APOC2
Uniprot No.:P02655
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:31.2 ng/mL-2000 ng/mL
灵敏度:15.6 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Metabolism
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 98 | ||||
Range % | 90-102 | |||||
1:200 | Average % | 88 | ||||
Range % | 82-92 | |||||
1:400 | Average % | 100 | ||||
Range % | 90-104 | |||||
1:800 | Average % | 101 | ||||
Range % | 91-105 |
回收率:
The recovery of human apo-C2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 99 | 90-102 | ||||
EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average20000.0880.0930.09110000.2150.2210.2185000.4150.4240.4202500.6930.6820.6881251.0951.0771.08662.51.5211.5001.51131.21.6681.5981.63302.0341.9872.011 |
货期:3-5 working days
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