Rat Pulmonary surfactant-associated protein A,SP-A ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08684r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠肺表面活性物质相关蛋白A(SP-A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPA1含量。SFTPA1是一种肺表面活性蛋白，属于C型凝集素亚家族，其基因突变与特发性肺纤维化有关。SFTPA1在表面活性蛋白稳态和抵抗呼吸道病原体方面发挥重要作用，其机制研究涉及基因突变、可溶性介质释放、细胞凋亡失调等过程。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。适用于研究呼吸系统疾病模型中SP-A的动态变化，如肺纤维化、慢性阻塞性肺疾病（COPD）的发病机制探索，或评估药物干预对肺表面活性物质代谢的影响本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Sftpa1 ELISA Kit; Sftp-1 ELISA Kit; Sftp1 ELISA Kit; Sftpa ELISA Kit; Pulmonary surfactant-associated protein A ELISA Kit; PSAP ELISA Kit; PSP-A ELISA Kit; SP-A ELISA Kit
缩写：
SFTPA1
Uniprot No.：
P08427
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml - 2000 pg/ml
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-99
1:2	Average %	93
	Range %	86-98
1:4	Average %	99
	Range %	95-104
1:8	Average %	97
	Range %	93-100

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.216	2.334	2.275	2.114
1000	1.808	1.767	1.788	1.626
500	1.365	1.328	1.347	1.185
250	0.971	0.939	0.955	0.794
125	0.652	0.621	0.637	0.475
62.5	0.429	0.453	0.441	0.280
31.25	0.287	0.284	0.286	0.124
0	0.165	0.158	0.162