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Human Serum amyloid A-4 protein(SAA4) ELISA kit

  • 中文名: 人血清淀粉样蛋白A4(SAA4)ELISA kit
货号: IPD-EL020659HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人血清淀粉样蛋白A4(SAA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的SAA4含量。试剂盒检测范围为15.6 ng/ml-1000 ng/ml,灵敏度为3.9 ng/ml。该产品适用于基础医学研究中SAA4蛋白的表达动态分析、疾病动物模型验证及炎症相关分子机制探索,可为生物标志物筛选、病理机制解析及药物干预效果评估提供可靠数据支持,严格限用于科研领域。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Amyloid A; serum; 4 ELISA Kit; C SAA ELISA Kit; C-SAA ELISA Kit; Constitutively expressed serum amyloid A protein ELISA Kit; CSAA ELISA Kit; SAA 4 ELISA Kit; Saa4 ELISA Kit; SAA4 protein ELISA Kit; SAA4_HUMAN ELISA Kit; Serum amyloid A 4 protein ELISA Kit; Serum amyloid A-4 protein ELISA Kit; Serum amyloid A4 constitutive ELISA Kit
  • 缩写:
    SAA4
  • Uniprot No.:
    P35542
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 ng/ml-1000 ng/ml.
  • 灵敏度:
    3.9 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human SAA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    97


    Range %

    93-101


    1:200

    Average %

    100


    Range %

    95-104


    1:400

    Average %

    95


    Range %

    89-99


    1:800

    Average %

    99


    Range %

    94-104


  • 回收率:

    The recovery of human SAA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-97


    EDTA plasma (n=4)

    92

    88-97


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.845

    2.765

    2.805

    2.702


    500

    2.147

    2.089

    2.118

    2.015


    250

    1.422

    1.437

    1.430

    1.327


    125

    0.730

    0.712

    0.721

    0.618


    62.5

    0.394

    0.376

    0.385

    0.282


    31.2

    0.237

    0.242

    0.240

    0.137


    15.6

    0.153

    0.147

    0.150

    0.047


    0

    0.101

    0.105

    0.103




  • 货期:
    3-5 working days

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