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A 96-well Assay plate --The 96-well plate has been pre-coated with human DBP.
Standard(Freeze-dried) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated DBP antibody(100 x concentrate) (1 x 60 μl) --Bind to the DBP, and HRP catalyzes the TMB to elicit a chromogenic reaction.
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated DBP antibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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