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Human Vitamin D-binding protein,DBP ELISA Kit

  • 中文名: 人维生素D结合蛋白(DBP)ELISA Kit
货号: IPD-E11859h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人维生素D结合蛋白(DBP)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GC含量。人维生素D结合蛋白(DBP)是一种多功能球蛋白,主要功能是结合和转运维生素D及其代谢物。研究发现,DBP在调节维生素D水平、维持骨骼健康、参与免疫调节等方面发挥重要作用。近年来,DBP在糖尿病肾病、肝癌等疾病中的表达异常及其与疾病进展的关系受到关注。试剂盒检测范围为0.3125 ng/ml- 20ng/ml,灵敏度为0.078 ng/ml。为科研领域研究维生素D代谢机制、评估营养干预效果、探索炎症相关疾病分子机制提供可靠工具,尤其适用于代谢组学、细胞功能实验及动物模型等基础研究场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    DBP ELISA Kit; DBP/GC ELISA Kit; GC ELISA Kit; Gc globulin ELISA Kit; Gc-globulin ELISA Kit; GRD3 ELISA Kit; Group specific component ELISA Kit; Group specific component vitamin D binding protein ELISA Kit; Group-specific component ELISA Kit; hDBP ELISA Kit; VDB ELISA Kit; VDBG ELISA Kit; VDBP ELISA Kit; Vitamin D binding alpha globulin ELISA Kit; Vitamin D-binding protein ELISA Kit; VTDB_HUMAN ELISA Kit
  • 缩写:
    GC
  • Uniprot No.:
    P02774
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.3125 ng/ml - 20ng/ml
  • 灵敏度:
    0.078 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 本试剂盒所含材料:


    • A 96-well Assay plate --The 96-well plate has been pre-coated with human DBP.

    • Standard(Freeze-dried) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.

    • HRP-conjugated DBP antibody(100 x concentrate) (1 x 60 μl) --Bind to the DBP, and HRP catalyzes the TMB to elicit a chromogenic reaction.

    • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated DBP antibody solution.

    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.

    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.

    • 本试剂盒不含材料:


      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.

      • An incubator that can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:
        3-5 working days

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