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Mouse Annexin A1(ANXA1) ELISA kit

  • 中文名： 小鼠膜联蛋白A1(ANXA1)酶联免疫试剂盒
货号: IPD-EL001836MO
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠膜联蛋白A1(ANXA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ANXA1含量。ANXA1即膜联蛋白A1，是一种钙依赖性磷脂结合蛋白。它参与细胞的多种生理过程，如炎症反应、细胞增殖等。研究机制方面，其可通过与细胞膜上的受体结合，调节细胞信号通路，在炎症和肿瘤等疾病中发挥重要作用，是潜在的治疗靶点。试剂盒检测范围为15.6 ng/ml- 1000 ng/ml，灵敏度为3.9 ng/ml。可广泛用于炎症相关疾病模型、免疫调控机制研究、肿瘤微环境分析等科研场景，例如评估药物干预对ANXA1表达水平的影响，或探索其在细胞信号传导中的作用，为分子机制研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Anxa1 ELISA kit; Anx1 ELISA kit; Lpc-1 ELISA kit; Lpc1Annexin A1 ELISA kit; Annexin I ELISA kit; Annexin-1 ELISA kit; Calpactin II ELISA kit; Calpactin-2 ELISA kit; Chromobindin-9 ELISA kit; Lipocortin I ELISA kit; Phospholipase A2 inhibitory protein ELISA kit; p35 ELISA kit
  • 缩写：
    ANXA1
  • Uniprot No.：
    P10107
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/ml - 1000 ng/ml
  • 灵敏度：
    3.9 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANXA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    97-111


    1:2

    Average %

    110


    Range %

    103-116


    1:4

    Average %

    98


    Range %

    91-105


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse ANXA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    103

    99-109


    EDTA plasma (n=4)

    96

    90-102


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.970

    2.834

    2.902

    2.724


    500

    2.458

    2.307

    2.382

    2.204


    250

    1.570

    1.528

    1.549

    1.371


    125

    0.843

    0.829

    0.836

    0.657


    62.5

    0.486

    0.491

    0.488

    0.310


    31.2

    0.362

    0.353

    0.358

    0.179


    15.6

    0.309

    0.294

    0.302

    0.123


    0

    0.182

    0.175

    0.178




  • 货期：
    3-5 working days

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