Human Fibulin-1(FBLN1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL008452HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人腓骨蛋白1(FBLN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FBLN1含量。FBLN1（fibulin-1）是一种分泌性糖蛋白，参与细胞外基质构建，与层粘连蛋白和巢蛋白结合，介导血小板粘附。研究显示，FBLN1在动脉粥样硬化、食管癌等疾病中表达增加，其表达受microRNA调控，可能通过影响细胞增殖、迁移等途径参与疾病发生发展。试剂盒检测范围为6.25 ug/ml-400 ug/ml，灵敏度为1.56 ug/ml。适用于科研领域中对FBLN1表达调控的机制研究、疾病模型中的蛋白动态分析以及生物样本中FBLN1含量的定量检测。为探索FBLN1在组织微环境重塑、疾病标志物筛选等方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Basement membrane protein 90 ELISA Kit; BM 90 ELISA Kit; FBLN ELISA Kit; Fbln1 ELISA Kit; FBLN1_HUMAN ELISA Kit; FIBL 1 ELISA Kit; FIBL-1 ELISA Kit; FIBL1 ELISA Kit; Fibulin 1 ELISA Kit; Fibulin-1 ELISA Kit
缩写：
FBLN1
Uniprot No.：
P23142
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
6.25 ug/ml-400 ug/ml
灵敏度：
1.56 ug/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-96
1:2	Average %	86
	Range %	82-95
1:4	Average %	93
	Range %	89-98
1:8	Average %	96
	Range %	92-101

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


μg/ml	OD1	OD2	Average	Corrected
400	2.698	2.556	2.627	2.497
200	1.667	1.540	1.604	1.474
100	0.953	0.970	0.962	0.832
50	0.576	0.573	0.575	0.445
25	0.449	0.458	0.454	0.324
12.5	0.351	0.353	0.352	0.222
6.25	0.252	0.260	0.256	0.126
0	0.131	0.129	0.130