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Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit

  • 中文名: 大鼠血管紧张素转化酶2(ACE2)ELISA Kit
货号: IPD-E14308r
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    大鼠血管紧张素转化酶2(ACE2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的ACE2含量。ACE2即血管紧张素转换酶 2,是肾素-血管紧张素系统的关键负调节因子。在研究机制方面,ACE2是新冠病毒的重要入侵靶点,它与病毒刺突蛋白结合介导病毒进入细胞,目前针对其开展的研究对新冠防治等有重要意义。试剂盒检测范围为0.02 ng/ml- 20 ng/ml,灵敏度为0.02 ng/ml。支持科研领域探究ACE2在疾病模型中的表达动态、药物干预效果评估或相关分子机制研究,为心血管、代谢及感染性疾病的基础研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Ace2Angiotensin-converting enzyme 2 ELISA kit; EC 3.4.17.23 ELISA kit; ACE-related carboxypeptidase) [Cleaved into: Processed angiotensin-converting enzyme 2] ELISA kit
  • 缩写:
    ACE2
  • Uniprot No.:
    Q5EGZ1
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.027 ng/ml-20 ng/ml
  • 灵敏度:
    0.027 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat ACE2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    99


    Range %

    94-103


    1:2

    Average %

    105


    Range %

    98-112


    1:4

    Average %

    93


    Range %

    89-98


    1:8

    Average %

    96


    Range %

    91-101


  • 回收率:

    The recovery of rat ACE2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    99

    94-105


    EDTA plasma (n=4)

    94

    88-100


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    20

    1.896

    1.934

    1.915

    1.824


    6.66

    1.134

    1.167

    1.151

    1.060


    2.22

    0.678

    0.708

    0.693

    0.602


    0.74

    0.483

    0.509

    0.496

    0.405


    0.24

    0.292

    0.301

    0.297

    0.206


    0.082

    0.171

    0.173

    0.172

    0.081


    0.027

    0.107

    0.112

    0.109

    0.018


    0

    0.089

    0.093

    0.091



  • 货期:
    3-5 working days

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