Pig free thyroxine (FT4) ELISA kit

货号: IPD-EQF027512PI

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：猪游离甲状腺素(FT4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FT4含量。FT4即游离甲状腺素，是甲状腺功能检查重要指标。它可直接反映甲状腺功能状态。研究机制方面，FT4参与机体代谢调节，其水平变化与甲状腺疾病如甲亢、甲减密切相关，检测它有助于准确诊断和病情判断。试剂盒检测范围为4 pmol/L-64 pmol/L，灵敏度为1.08 pmol/L。科研人员可将其应用于动物营养代谢研究、环境应激评估、甲状腺相关疾病模型构建等领域，尤其适用于需要动态监测甲状腺功能变化的实验设计，如激素分泌调控、代谢性疾病机制研究或毒理学评价等，为畜牧兽医研究及生物医学基础科研提供标准化检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：FT4
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：4 pmol/L-64 pmol/L
灵敏度：1.08 pmol/L
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	87-106
1:4	Average %	96
	Range %	90-110
1:8	Average %	91
	Range %	86-98

回收率：

The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-103
EDTA plasma (n=4)	96	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/LOD1OD2Average　 42.7712.7362.754　 81.4591.4421.451　 160.8270.8650.846　 320.5750.5650.570　 640.3180.3070.313

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