首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒,为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL2含量。CCL2，即单核细胞趋化蛋白1（MCP-1），属于CC趋化因子家族，主要在炎症免疫反应、病毒感染和肿瘤发生中起作用。其通过结合CCR2受体，诱导单核细胞、巨噬细胞和T淋巴细胞迁移，参与炎症反应和肿瘤转移。CCL2在多种疾病如肿瘤、神经炎症和糖尿病中发挥关键作用。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于炎症相关动物模型研究，如关节炎、神经退行性疾病模型的分子机制探索，亦可应用于药物干预实验或免疫调节研究中MCP - 1表达水平的动态监测，为心血管疾病、肿瘤免疫等领域的科研工作提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccl2 ELISA Kit; Je ELISA Kit; Mcp1 ELISA Kit; Scya2 ELISA Kit; C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Platelet-derived growth factor-inducible protein JE ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：CCL2
Uniprot No.：P10148
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||||||||
| Sample | Serum(n=4) | |||||||||||
| 1:1 | Average % | 88 | ||||||||||
| Range % | 82-94 | |||||||||||
| 1:2 | Average % | 95 | ||||||||||
| Range % | 91-98 | |||||||||||
| 1:4 | Average % | 102 | ||||||||||
| Range % | 96-108 | |||||||||||
| 1:8 | Average % | 95 | ||||||||||
| Range % | 89-98 | |||||||||||
回收率：
| The recovery of mouse MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||||||||
| Sample Type | Average % Recovery | Range | ||||||||||
| Serum (n=5) | 88 | 82-94 | ||||||||||
| EDTA plasma (n=4) | 97 | 92-104 | ||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||
| pg/mlOD1OD2AverageCorrected 50001.9461.8641.9051.806 25001.2941.4141.3541.255 12500.8610.8430.8520.753 6250.5550.5460.5510.452 3120.3470.3560.3520.253 1560.2670.2580.2630.164 780.1710.1790.1750.076 00.0990.0980.099 | ||||||||||||
货期：3-5 working days
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