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产品描述：绵羊三碘甲状腺原氨酸(T3)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的T3含量。试剂盒检测范围为0.5 ng/mL-8 ng/mL，灵敏度为0.5 ng/mL。适用于科研领域中对甲状腺激素代谢调控机制的研究；适用于动物生理学研究中甲状腺功能评估、内分泌干扰物效应分析、药物干预实验中的激素水平监测，或体外细胞模型中甲状腺激素分泌机制研究；为探究甲状腺相关代谢通路、疾病模型构建及药理学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：T3
种属：Ovis aries (Sheep)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.5 ng/mL-8 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheepT3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 90-100 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 85-96 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 82-99 | ||||||
回收率：
| The recovery of sheep T3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 85-102 | |||||
| EDTA plasma (n=4) | 96 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 80.1720.1650.169 40.2890.2780.284 20.5490.5570.553 11.2521.2631.258 0.52.1242.1412.133 | |||||||
货期：3-5 working days
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