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Mouse Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

  • 中文名： 小鼠上皮中性粒细胞活化肽78(ENA-78/CXCL5)ELISA Kit
货号: IPD-E08180m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠上皮中性粒细胞活化肽78(ENA-78/CXCL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL5含量。CXCL5是一种趋化因子，在肿瘤微环境、炎症反应等方面有重要作用。研究表明，它可招募特定免疫细胞，参与肿瘤血管生成、侵袭转移等过程。以CXCL5为靶点，有望从调控其表达、阻断相关信号通路等方面开发治疗策略。试剂盒检测范围为62.5 pg/ml- 4000 pg/ml，灵敏度为15.6 pg/ml。适用于炎症机制研究、疾病模型（如肺炎、关节炎或肿瘤模型）中趋化因子动态变化的分析，以及药物干预实验中生物标志物的定量评估。科研人员可通过该产品探究CXCL5在免疫调控、病理进程及潜在治疗靶点中的功能，为相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5) [Cleaved into: GCP-2(1-78); GCP-2(9-78)] ELISA Kit
  • 缩写：
    CXCL5
  • Uniprot No.：
    P50228
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/ml - 4000 pg/ml
  • 灵敏度：
    15.6 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%








    Three samples of known concentration were tested twenty times on one plate to assess.






    Inter-assay Precision (Precision between assays): CV%<10%








    Three samples of known concentration were tested in twenty assays to assess.








    Intra-Assay Precision

    Inter-Assay Precision





    Sample

    1

    2

    3

    1

    2

    3





    n

    20

    20

    20

    20

    20

    20





    Mean(pg/ml)

    516.646

    497.903

    491.373

    484.723

    483.883

    477.090





    SD

    0.035

    0.033

    0.038

    0.041

    0.041

    0.038





    CV(%)

    7.172

    6.286

    7.350

    8.055

    8.039

    7.600





    TYPICAL DATA

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.





    pg/ml

    OD1

    OD2

    Average

    Corrected







    4000

    2.88

    2.876

    2.878

    2.726







    2000

    2.239

    2.235

    2.237

    2.085







    1000

    1.263

    1.259

    1.261

    1.109







    500

    0.646

    0.642

    0.644

    0.492







    250

    0.342

    0.338

    0.340

    0.188







    125

    0.249

    0.245

    0.247

    0.095







    62.5

    0.218

    0.214

    0.216

    0.064

    0.064

    0

    0.154

    0.150

    0.152











  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.






    Sample

    Serum(n=4)



    1:20

    Average %

    88



    Range %

    83-91



    1:40

    Average %

    104



    Range %

    98-108



    1:80

    Average %

    92



    Range %

    87-96



    1:160

    Average %

    96



    Range %

    91-99










  • 回收率：

    The recovery of mouse ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.





    Sample Type

    Average % Recovery

    Range%



    Serum (n=5)

    90

    84-94



    EDTA plasma (n=4)

    94

    87-97











  • 货期：
    3-5 working days

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