Horse Progesterone(PROG) ELISA kit

大包装询价

货号: IPD-E13183Hs

Price： ￥3000

48T 96T

数量：

大包装询价

产品描述：
马孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PROG含量。试剂盒检测范围为0.25 ng/mL-100 ng/mL，灵敏度为0.25 ng/mL。本试剂盒采用高特异性抗体，可精准识别样本中的孕酮分子，适用于科研场景下的马科动物生殖生理研究、妊娠状态追踪、繁殖效率评估及激素代谢分析。实验流程经优化设计，配套标准品及反应体系完备，可在常规实验室条件下快速完成检测，为畜牧业繁殖优化、野生动物保护等领域的激素水平研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
PROG
种属：
Equus caballus (Horse)
样本类型：
serum, plasma
检测范围：
0.25 ng/mL-100 ng/mL
灵敏度：
0.25 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-107
1:2	Average %	89
	Range %	83-99
1:4	Average %	95
	Range %	85-103
1:8	Average %	90
	Range %	86-102

回收率：

The recovery of horse PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-106
EDTA plasma (n=4)	95	82-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average
100	0.162	0.158	0.160
18.75	0.320	0.332	0.326
3.125	0.542	0.563	0.553
0.875	0.971	0.944	0.958
0.25	1.503	1.485	1.494
0	2.192	2.127	2.160

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.