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Fish Progesterone(PROG) ELISA Kit

  • 中文名: 鱼孕激素/孕酮(PROG)ELISA Kit
货号: IPD-E17295Fh
Price: ¥3000
数量:
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产品详情

  • 产品描述:
    鱼孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的PROG含量。靶点研究是药物研发的关键,涉及寻找疾病相关分子或细胞结构作为治疗目标。研究机制包括基因组学、蛋白质组学和生物信息学分析,以及高通量筛选技术。靶点发现有助于开发更精确的药物,提高治疗效果。试剂盒检测范围为0.15 ng/ml-70 ng/ml,灵敏度为0.2 ng/ml.。本产品适用于科研场景中鱼类生殖周期监测、环境内分泌干扰物效应评估、水产养殖繁殖管理优化等研究,兼容多种生物样本类型,操作便捷且重复性良好,为实验室开展鱼类激素相关机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    PROG
  • 种属:
    Fish
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    0.15 ng/ml-70 ng/ml
  • 灵敏度:
    0.2 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of fish PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    85-106


    1:2

    Average %

    90


    Range %

    89-99


    1:4

    Average %

    95


    Range %

    85-103


    1:8

    Average %

    90


    Range %

    87-103


  • 回收率:

    The recovery of fish PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    82-107


    EDTA plasma (n=4)

    96

    88-102


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    70

    0.143

    0.148

    0.146



    20

    0.279

    0.264

    0.272



    5

    0.523

    0.545

    0.534



    1

    0.861

    0.893

    0.877



    0.15

    1.428

    1.459

    1.444



    0

    2.113

    2.132

    2.123




  • 货期:
    3-5 working days

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