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Canine Progesterone,PROG ELISA kit

  • 中文名: 犬孕激素/孕酮(PROG)ELISA kit
货号: IPD-E07285c
Price: ¥3000
数量:
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产品详情

  • 产品描述:
    犬孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的PROG含量。试剂盒检测范围为0.15 ng/ml-70 ng/ml,灵敏度为0.2 ng/ml。适用于动物繁殖生理研究、生殖内分泌机制探索、药物对卵巢功能影响评估等场景,尤其可为犬类人工授精时机判断、妊娠监测及生殖疾病模型构建提供可靠数据支持本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    PROG
  • 种属:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    0.15 ng/ml-70 ng/ml.
  • 灵敏度:
    0.2 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of canine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    96


    Range %

    92-104


    1:2

    Average %

    94


    Range %

    91-99


    1:4

    Average %

    102


    Range %

    98-105


    1:8

    Average %

    89


    Range %

    82-94


  • 回收率:

    The recovery of canine PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    90-97


    EDTA plasma (n=4)

    101

    98-105


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    0

    2.284

    2.275

    2.280



    0.15

    1.429

    1.589

    1.509



    1

    0.843

    0.874

    0.859



    5

    0.566

    0.585

    0.576



    20

    0.362

    0.353

    0.358



    70

    0.201

    0.202

    0.202




  • 货期:
    3-5 working days

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