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Bovine progesterone,PROG ELISA Kit

  • 中文名： 牛孕激素/孕酮(PROG)ELISA Kit
货号: IPD-E08172b
Price： ￥3000
数量：
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产品详情

  • 产品描述：
    牛孕激素/孕酮(PROG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PROG含量。试剂盒检测范围为1 ng/ml-70 ng/ml，灵敏度为0.2 ng/mL。广泛应用于畜牧业科研中母牛繁殖周期监测、妊娠诊断辅助研究、繁殖障碍机制分析及激素调控实验，为优化繁殖管理策略提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    PROG
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    1 ng/ml-70 ng/ml.
  • 灵敏度：
    0.2 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<15%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    92-106


    1:2

    Average %

    95


    Range %

    91-99


    1:4

    Average %

    89


    Range %

    85-94


    1:8

    Average %

    85


    Range %

    80-89


  • 回收率：

    The recovery of bovine PROG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    88-98


    EDTA plasma (n=4)

    102

    98-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average



    0

    2.321

    2.362

    2.342



    1

    1.907

    1.845

    1.876



    3

    1.321

    1.479

    1.400



    10

    0.863

    0.893

    0.878



    30

    0.561

    0.542

    0.552



    70

    0.294

    0.309

    0.301




  • 货期：
    3-5 working days

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