首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人催乳素(PRL/LTH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的PRL含量。PRL即泌乳素，它是腺垂体分泌的多肽蛋白激素。其分泌受下丘脑调节。在生理上，PRL参与乳腺发育、乳汁生成等。研究机制方面，主要聚焦其信号通路，如JAK-STAT通路，异常的PRL水平与多种疾病相关，像高泌乳素血症等。试剂盒检测范围为40 μIU/mL-2000 μIU/mL，灵敏度为50 μIU/mL。可为内分泌调控机制研究、生殖生理学实验、肿瘤微环境分析等科研领域提供可靠数据支持；特别适用于体外细胞模型分泌水平监测、动物疾病模型激素动态变化分析以及临床前药物干预效果评估等场景，满足科研工作者对催乳素分子功能探索及病理生理机制解析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
缩写：PRL
Uniprot No.：P01236
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：40 μIU/mL-2000 μIU/mL
灵敏度：50 μIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-102 | ||||
| EDTA plasma (n=4) | 97 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μIU/mlOD1OD2AverageCorrected 20002.1862.1052.1462.115 8001.2011.1971.1991.168 3000.6220.6190.6210.590 1000.2960.3020.2990.268 400.1450.1480.1470.116 00.0320.0300.031 | ||||||
货期：3-5 working days
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