Mouse Leptin receptor,LR/Ob-R ELISA KIT

中文名： 小鼠苗条素受体(LR/Ob-R)ELISA Kit

货号: IPD-E04648m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠苗条素受体(LR/Ob-R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEPR含量。LEPR即瘦素受体，其背景在于它是瘦素发挥生物学效应的关键分子。研究机制方面，瘦素与LEPR结合后，能激活下游信号通路，参与能量代谢、食欲调节等过程。对LEPR深入研究，有助于揭示肥胖等代谢疾病的发病机制并开发相关疗法。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，灵敏度为0.039 ng/ml。适用于细胞模型、动物实验中瘦素信号通路机制探索、代谢调控药物筛选及相关病理生理学研究。为肥胖相关基础研究与药物开发提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Lepr ELISA Kit; Db ELISA Kit; ObrLeptin receptor ELISA Kit; LEP-R ELISA Kit; B219 ELISA Kit; OB receptor ELISA Kit; OB-R ELISA Kit; CD antigen CD295 ELISA Kit
缩写：
LEPR
Uniprot No.：
P48356
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 ng/ml- 10 ng/ml
灵敏度：
0.039 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)1.2581.2661.2491.2621.2401.250SD0.0370.0410.0400.0640.0590.069CV(%)3.8114.2094.1326.5826.1157.126

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

105

Range %

100-110

1:2

Average %

Range %

94-102

1:4

Average %

Range %

86-94

1:8

Average %

Range %

88-96

回收率：

The recovery of mouse LR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	82-90
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
10	2.022	2.128	2.075	1.916
5	1.653	1.603	1.628	1.469
2.5	1.260	1.295	1.278	1.119
1.25	0.981	0.954	0.968	0.809
0.625	0.739	0.719	0.729	0.570
0.312	0.447	0.461	0.454	0.295
0.156	0.284	0.287	0.286	0.127
0	0.156	0.162	0.159