| WB | 1/500-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | AOP1, MER5, AOP-1, SP-22, HBC189, PRO1748, prx-III |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PRDX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PRDX3抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Peroxiredoxin 3 Is a Redox-Dependent Target of Thiostrepton in Malignant Mesothelioma Cells*
**作者**：C. Riganti et al.
**摘要**：该研究揭示了PRDX3在恶性间皮瘤中的抗氧化作用，通过使用PRDX3抗体检测其表达水平，发现硫链丝霉素通过抑制PRDX3活性增强氧化应激，从而抑制肿瘤细胞生长。
2. **文献名称**：*PRDX3 Supports Mitochondrial Stress Adaptation in Neurodegeneration*
**作者**：A. M. Pickrell et al.
**摘要**：文章利用PRDX3抗体分析其在神经元线粒体中的定位，表明PRDX3通过清除活性氧保护神经元免受氧化损伤，并参与帕金森病等神经退行性疾病的病理调控。
3. **文献名称**：*PRDX3 as a Prognostic Biomarker in Gastric Cancer*
**作者**：Y. Li et al.
**摘要**：该研究通过免疫组化(使用PRDX3抗体)发现，PRDX3在胃癌组织中高表达且与患者不良预后相关，提示其可作为潜在治疗靶点和预后标志物。
(注：以上文献信息为示例，实际引用需以具体论文为准。)
**Background of PRDX3 Antibody**
Peroxiredoxin-3 (PRDX3), a member of the peroxiredoxin family, is a mitochondrial antioxidant enzyme critical for neutralizing reactive oxygen species (ROS) and maintaining redox homeostasis. It plays a vital role in protecting mitochondrial components from oxidative damage, regulating apoptosis, and modulating cellular proliferation. PRDX3 is encoded by the *PRDX3* gene and is predominantly localized to the mitochondria, distinguishing it from other cytosolic peroxiredoxins.
PRDX3 antibodies are immunological tools designed to detect and quantify PRDX3 protein expression in research applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are pivotal in studying PRDX3's involvement in oxidative stress-related pathologies, including neurodegenerative diseases, cancer, cardiovascular disorders, and diabetes. For instance, PRDX3 overexpression has been linked to cancer progression and chemoresistance, while its deficiency correlates with mitochondrial dysfunction and accelerated aging.
Structurally, PRDX3 functions as a homodimer, utilizing cysteine residues to catalyze peroxide reduction. Antibodies targeting PRDX3 often recognize specific epitopes, such as its C-terminal or catalytic domains, enabling researchers to assess its expression levels, subcellular localization, and post-translational modifications. Validation of PRDX3 antibodies is essential to ensure specificity, as cross-reactivity with other peroxiredoxin isoforms (e.g., PRDX1 or PRDX2) can compromise experimental accuracy.
Overall, PRDX3 antibodies are indispensable for exploring mitochondrial oxidative stress mechanisms, disease biomarkers, and therapeutic targets, bridging molecular insights with clinical applications.
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