Mouse Leptin,LEP ELISA kit

中文名： 小鼠瘦素(LEP)ELISA Kit

货号: IPD-E04650m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEP含量。LEP即瘦素，其背景在于它是脂肪组织分泌的一种蛋白质类激素。研究机制方面，它能作用于下丘脑的受体，调节能量代谢和食欲。通过向中枢神经系统传递脂肪储存信号，抑制食欲、增加能量消耗，对体重和代谢稳态维持起重要作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。科研人员可将其应用于肥胖模型小鼠的代谢机制研究、药物干预后LEP表达水平动态监测，或探究不同组织（如脂肪、肝脏）中瘦素分泌的调控通路，为能量代谢相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Lep ELISA Kit; Ob ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
缩写：
LEP
Uniprot No.：
P41160
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)2.4582.5692.5162.5132.5202.523SD0.0320.0310.0340.0500.0480.053CV(%)4.6654.3854.8787.1806.8797.590

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

86-95

1:2

Average %

103

Range %

97-105

1:4

Average %

Range %

87-95

1:8

Average %

Range %

92-100

回收率：

The recovery of mouse LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	95	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
20	2.196	2.184	2.190	2.040
10	1.641	1.628	1.635	1.485
5	1.118	1.103	1.111	0.961
2.5	0.686	0.707	0.697	0.547
1.25	0.415	0.403	0.409	0.259
0.625	0.285	0.276	0.281	0.131
0.312	0.202	0.214	0.208	0.058
0	0.152	0.148	0.150