首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒

Rat Leptin,LEP ELISA kit

  • 中文名: 大鼠瘦素(LEP)ELISA kit
货号: IPD-E07433r
Price: ¥2500
数量:
大包装询价

产品详情

  • 产品描述:
    大鼠瘦素(LEP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆.样本中的LEP含量。LEP(瘦素)是一种由脂肪组织分泌的激素,作为靶点备受关注。其研究机制主要是通过与下丘脑的受体结合,调节食欲和能量消耗。研究该靶点有助于深入了解肥胖、代谢综合征等疾病病理,为相关药物研发提供方向。试剂盒检测范围为0.78 ng/ml-50 ng/ml.,灵敏度为0.195 ng/ml.。适用于肥胖机制研究、代谢综合征动物模型评估、营养干预实验等科研场景,适用于长期实验中的纵向数据采集或不同组织来源样本的LEP表达水平对比分析,为研究能量代谢调控、激素互作网络提供可靠的实验工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Lep ELISA Kit; Ob ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
  • 缩写:
    LEP
  • Uniprot No.:
    P50596
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates.
  • 检测范围:
    0.78 ng/ml-50 ng/ml.
  • 灵敏度:
    0.195 ng/ml.
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    80-92


    1:2

    Average %

    95


    Range %

    91-105


    1:4

    Average %

    94


    Range %

    89-97


    1:8

    Average %

    90


    Range %

    86-96


  • 回收率:

    The recovery of rat LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    87-94


    EDTA plasma (n=4)

    99

    94-107


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.325

    2.434

    2.379

    2.199


    25

    1.578

    1.667

    1.623

    1.442


    12.5

    0.836

    0.928

    0.882

    0.701


    6.25

    0.499

    0.529

    0.514

    0.333


    3.12

    0.366

    0.351

    0.358

    0.178


    1.56

    0.286

    0.293

    0.290

    0.109


    0.78

    0.227

    0.234

    0.231

    0.050


    0

    0.177

    0.185

    0.181




  • 货期:
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×