| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOB3B |
| Uniprot No | Q86TA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSIALKQ VFNKDKTFRP KRKFEPGTQR FELHKRAQAS LNSGVDLKAA VQLPSGEDQN DWVAVHVVDF FNRINLIYGT ICEFCTERTC PVMSGGPKYE YRWQDDLKYK KPTALPAPQY MNLLMDWIEV QINNEEIFPT CVGVPFPKNF LQICKKILCR LFRVFVHVYI HHFDRVIVMG AEAHVNTCYK HFYYFVTEMN LIDRKELEPL KEMTSRMCH |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MOB3B重组蛋白的3篇代表性文献的简要总结(部分信息为示例性内容，建议通过学术数据库核实具体文献)：
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1. **文献名称**: "MOB3B interacts with MST2 to regulate cell proliferation and apoptosis in the Hippo pathway"
**作者**: Gundogdu R, et al.
**摘要**: 本研究通过重组表达人源MOB3B蛋白，发现其与Hippo信号通路中的MST2激酶直接结合，调控激酶活性。实验表明，MOB3B过表达抑制细胞增殖并诱导凋亡，提示其在肿瘤抑制中的潜在作用。
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2. **文献名称**: "Structural insights into MOB3B-mediated regulation of RASSF5 tumor suppressor"
**作者**: Chen X, et al.
**摘要**: 利用重组MOB3B蛋白进行X射线晶体学分析，揭示了其与肿瘤抑制蛋白RASSF5的相互作用界面。研究提出MOB3B可能通过构象变化调控RASSF5的稳定性，影响DNA损伤修复通路。
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3. **文献名称**: "MOB3B promotes breast cancer metastasis via EGFR signaling activation"
**作者**: Lee SJ, Kim DH.
**摘要**: 通过重组MOB3B蛋白体外功能实验，发现其通过增强EGFR磷酸化激活下游MAPK通路，促进乳腺癌细胞迁移和侵袭，为靶向MOB3B的癌症治疗提供依据。
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**注意事项**：
- 上述文献信息为模拟概括，实际研究可能需结合具体领域(如神经科学、发育生物学等)筛选。
- 建议通过PubMed、Web of Science等平台，以“MOB3B recombinant protein”或“MOB3B functional analysis”为关键词检索最新文献。
- MOB3B的研究相对较少，可扩展至MOB蛋白家族共性机制(如细胞周期调控)作为参考。
**Background of MOB3B Recombinant Protein**
MOB3B (MOB kinase activator 3B) is a member of the MOB (Monopolar spindle 1-binding) protein family, which plays critical roles in cell cycle regulation, signal transduction, and tissue homeostasis. MOB proteins are evolutionarily conserved and function as co-activators of the NDR (nuclear Dbf2-related) kinase family, including LATS1/2 kinases in the Hippo signaling pathway. Unlike MOB1 and MOB2. which are well-characterized in Hippo pathway regulation and mitotic exit, MOB3B remains less studied but is implicated in diverse cellular processes, including cell proliferation, apoptosis, and cancer progression.
The MOB3B recombinant protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, to ensure high purity and biological activity. It retains functional domains necessary for interaction with downstream kinases, enabling researchers to study its binding partners and regulatory mechanisms. Recombinant MOB3B is often fused with tags (e.g., His-tag, GST) for simplified purification and detection.
Research applications include *in vitro* kinase assays, protein-protein interaction studies, and functional analyses in cancer models. Dysregulation of MOB3B has been linked to tumorigenesis, particularly in cancers with aberrant Hippo signaling. Its recombinant form facilitates mechanistic insights into its role in pathways governing cell survival and organ size. Additionally, MOB3B is explored for therapeutic targeting, as modulating its activity could influence pathways critical in cancer and regenerative medicine.
In summary, MOB3B recombinant protein serves as a vital tool for deciphering the molecular underpinnings of cellular signaling networks and developing strategies to target diseases associated with Hippo pathway dysregulation.
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