| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP8 |
| Uniprot No | P22894 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 101-467aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSLTPGNPKWERTNLTYRIRNYTPQLSEA EVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPN GILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAH SSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPK PCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPT GIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAI DAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAV FQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP8重组蛋白的3篇参考文献示例(内容为模拟虚构，仅供参考)：
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1. **标题**: *"High-yield expression and functional characterization of recombinant human MMP-8 in a baculovirus-insect cell system"*
**作者**: Müller, A., et al.
**摘要**: 该研究利用杆状病毒-昆虫细胞系统高效表达重组人MMP8.并通过亲和层析纯化获得高纯度蛋白。酶活性实验表明，重组MMP8能特异性切割胶原蛋白Ⅰ/Ⅱ/Ⅲ，为研究其在组织重塑中的作用提供了工具。
2. **标题**: *"Recombinant MMP-8 modulates neutrophil extracellular traps (NETs) in acute lung injury models"*
**作者**: Chen, L., et al.
**摘要**: 研究团队通过哺乳动物细胞表达重组MMP8.发现其能降解中性粒细胞胞外陷阱(NETs)的关键成分，减轻小鼠急性肺损伤模型的炎症反应，提示MMP8在调控先天免疫中的潜在治疗价值。
3. **标题**: *"Structural basis of substrate specificity in recombinant MMP-8: Insights from mutagenesis and molecular dynamics"*
**作者**: Tanaka, K., et al.
**摘要**: 通过定点突变和分子动力学模拟，本文解析了重组MMP8催化结构域的关键氨基酸残基如何影响其底物选择性，为设计特异性MMP8抑制剂提供了结构基础。
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以上文献示例聚焦于MMP8重组蛋白的表达系统、疾病相关功能机制及结构分析，涵盖基础研究与潜在应用方向。如需真实文献，建议通过PubMed或Google Scholar检索关键词(如"recombinant MMP8 expression"或"MMP8 collagenase activity")。
Matrix metalloproteinase 8 (MMP8), also known as collagenase-2 or neutrophil collagenase, is a member of the MMP family of zinc-dependent endopeptidases. It plays a critical role in extracellular matrix (ECM) remodeling by degrading collagen types I, II, and III, as well as other substrates. Primarily secreted by neutrophils, MMP8 is involved in inflammatory responses, tissue repair, and pathological processes such as cancer metastasis, arthritis, and fibrosis. Its activity is tightly regulated at transcriptional, post-translational, and inhibitory levels, particularly by tissue inhibitors of metalloproteinases (TIMPs).
Recombinant MMP8 protein is produced using heterologous expression systems (e.g., E. coli, mammalian, or insect cells) to enable controlled studies of its enzymatic functions. The recombinant form typically includes the catalytic domain and may retain propeptide regions that require proteolytic cleavage for activation. Advanced purification techniques like affinity chromatography ensure high purity and activity.
As a research tool, recombinant MMP8 facilitates investigations into ECM dynamics, neutrophil biology, and disease mechanisms. It is widely used in drug discovery to screen MMP8-specific inhibitors or to validate therapeutic targets in inflammatory diseases and cancer. Interestingly, MMP8 exhibits dual roles in cancer progression, showing both tumor-promoting and protective effects depending on context, making its recombinant form valuable for clarifying these paradoxical functions. Current studies also explore its potential as a diagnostic biomarker or therapeutic agent in tissue engineering applications.
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