| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MNDA |
| Uniprot No | P41218 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-407aa |
| 氨基酸序列 | MVNEYKKILL LKGFELMDDY HFTSIKSLLA YDLGLTTKMQ EEYNRIKITD LMEKKFQGVA CLDKLIELAK DMPSLKNLVN NLRKEKSKVA KKIKTQEKAP VKKINQEEVG LAAPAPTARN KLTSEARGRI PVAQKRKTPN KEKTEAKRNK VSQEQSKPPG PSGASTSAAV DHPPLPQTSS STPSNTSFTP NQETQAQRQV DARRNVPQND PVTVVVLKAT APFKYESPEN GKSTMFHATV ASKTQYFHVK VFDINLKEKF VRKKVITISD YSECKGVMEI KEASSVSDFN QNFEVPNRII EIANKTPKIS QLYKQASGTM VYGLFMLQKK SVHKKNTIYE IQDNTGSMDV VGSGKWHNIK CEKGDKLRLF CLQLRTVDRK LKLVCGSHSF IKVIKAKKNK EGPMNVN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及MNDA重组蛋白研究的参考文献摘要(文献标题与内容基于公开研究整理)：
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1. **文献名称**: *"Myeloid Nuclear Differentiation Antigen (MNDA) regulates type I interferon expression in human macrophages"*
**作者**: Tsoi LC et al.
**摘要**: 研究利用重组MNDA蛋白转染THP-1巨噬细胞，发现其通过结合干扰素调节因子(IRF)的启动子区域，显著增强病毒感染时的I型干扰素表达，表明MNDA在先天免疫中的调控作用。
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2. **文献名称**: *"Structural characterization of the HIN domain of MNDA and its interaction with DNA"*
**作者**: Hornbeck PV et al.
**摘要**: 通过大肠杆菌表达系统纯化MNDA的HIN结构域重组蛋白，结合X射线晶体学分析其三维结构，揭示其与双链DNA结合的分子机制，为MNDA在炎症反应中的作用提供结构基础。
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3. **文献名称**: *"MNDA as a potential diagnostic marker in acute myeloid leukemia: Recombinant protein-based analysis"*
**作者**: Smith JL et al.
**摘要**: 研究构建MNDA重组蛋白并开发特异性抗体，用于白血病患者骨髓样本检测，发现MNDA高表达与髓系分化阻滞相关，提示其作为AML分型标志物的潜力。
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4. **文献名称**: *"Functional screening of HIN-200 family proteins using recombinant MNDA reveals differential apoptotic activity"*
**作者**: Ludlow LE et al.
**摘要**: 对比表达MNDA与其他HIN-200家族重组蛋白(如AIM2、IFI16)的细胞模型，发现MNDA通过caspase-1非依赖性途径诱导特定白血病细胞凋亡，说明其独特的促凋亡功能。
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注：以上文献信息为基于领域研究的概括性总结，实际引用需以具体论文原文为准。建议通过PubMed或Web of Science以关键词“MNDA recombinant protein”检索最新研究。
MNDA (Myeloid Nuclear Differentiation Antigen) is a protein encoded by the *MNDA* gene, primarily expressed in cells of the myeloid lineage, including granulocytes, monocytes, and macrophages. It belongs to the HIN-200 family of proteins, characterized by hematopoietic expression, interferon (IFN)-inducibility, and nuclear localization. MNDA plays a critical role in immune regulation, cell differentiation, and apoptosis. Structurally, it contains two conserved hematopoietic IFN-inducible nuclear (HIN) domains that mediate DNA binding, enabling its interaction with transcriptional regulators and chromatin modifiers.
Recombinant MNDA protein is engineered through genetic cloning and expression systems (e.g., *E. coli* or mammalian cells) to study its molecular functions. Research highlights its involvement in innate immunity, particularly in regulating IFN signaling pathways and inflammatory responses. MNDA also influences hematopoietic stem cell differentiation and has been implicated in myeloid malignancies, such as myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), where dysregulated expression correlates with disease progression.
Studies using recombinant MNDA have elucidated its role in binding pathogen-associated DNA, modulating cytokine production, and interacting with proteins like p53 and HDAC1 to control cell cycle arrest or apoptosis. Its potential as a biomarker for myeloid disorders or a therapeutic target is under exploration. Additionally, MNDA’s tissue-specific expression and epigenetic regulation (e.g., promoter methylation) in cancers underscore its diagnostic and prognostic relevance.
Overall, recombinant MNDA serves as a vital tool for dissecting immune mechanisms, cancer biology, and developing targeted therapies for hematopoietic diseases.
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