| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOB1B |
| Uniprot No | Q7L9L4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSFLFGS RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MOB1B重组蛋白研究的模拟参考文献示例(注：部分内容为示例性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: *"MOB1B phosphorylation by MST kinases regulates the Hippo-YAP signaling pathway"*
**作者**: Hergovich, A., et al.
**摘要**: 本研究利用重组人源MOB1B蛋白，揭示了其被MST1/2激酶磷酸化的关键位点(Thr35)，并证明此修饰对Hippo通路中LATS激酶激活及YAP/TAZ抑制至关重要。重组蛋白通过大肠杆菌表达系统纯化，并用于体外激酶实验。
2. **文献名称**: *"Structural basis for MOB1B recognition by the tumor suppressor RASSF1A"*
**作者**: Praskova, M., et al.
**摘要**: 通过X射线晶体学解析重组MOB1B蛋白与RASSF1A的复合物结构，阐明二者结合界面，证明MOB1B在细胞凋亡中的调控作用。重组蛋白在昆虫细胞中表达，采用亲和层析纯化。
3. **文献名称**: *"Recombinant MOB1B enhances LATS kinase activity in vitro and suppresses tumor growth in vivo"*
**作者**: Kim, S.Y., & Park, J.
**摘要**: 开发了一种高效的真核表达系统(哺乳动物HEK293细胞)制备重组MOB1B蛋白，证实其与LATS1激酶结合后显著增强后者对YAP的磷酸化能力，并在小鼠模型中验证其抗肿瘤效果。
4. **文献名称**: *"Purification and functional characterization of MOB1B in Drosophila cell cycle regulation"*
**作者**: Lai, Z.C., et al.
**摘要**: 利用杆状病毒系统表达并纯化果蝇MOB1B重组蛋白，发现其通过调控Dco(LATS同源物)影响细胞周期进程，为Hippo通路的进化保守性提供证据。
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**建议**：实际研究中可通过PubMed、Web of Science等平台，以关键词“MOB1B recombinant protein”、“MOB1B Hippo pathway”检索最新文献，重点关注蛋白互作、结构生物学或疾病模型研究。
MOB1B is a member of the MOB (Mps One Binder) protein family, which plays critical roles in cell cycle regulation, mitotic progression, and signal transduction pathways. As a core component of the Hippo tumor suppressor pathway, MOB1B interacts with serine/threonine kinases MST1/2 (mammalian STE20-like kinases 1/2) to regulate the activity of downstream effectors LATS1/2 (large tumor suppressor kinases 1/2). This interaction is essential for maintaining tissue homeostasis and controlling organ size by balancing cell proliferation and apoptosis.
Structurally, MOB1B contains conserved Mob1/phocein domains that mediate binding to NDR (nuclear Dbf2-related) kinases and other regulatory partners. It shares high homology with MOB1A, its paralog, but exhibits distinct expression patterns and functional nuances in specific cellular contexts. Dysregulation of MOB1B has been implicated in various cancers, where it may act as either a tumor suppressor or oncogenic modulator depending on cellular conditions and post-translational modifications.
Recombinant MOB1B protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling researchers to study its biochemical properties, interaction networks, and regulatory mechanisms in vitro. The purified protein retains the ability to form complexes with Hippo pathway components, making it valuable for structural studies (e.g., X-ray crystallography), enzymatic assays, and drug discovery initiatives targeting this pathway. Its recombinant form also serves as a critical tool for investigating mitotic errors, chromosomal instability, and potential therapeutic strategies for cancers with Hippo pathway alterations. Recent studies further suggest roles in autophagy regulation and cilia biology, expanding its biomedical relevance beyond traditional pathway mapping.
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