| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MLC1F; MLC3F |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MYL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MYL12B抗体的参考文献示例(注：以下内容为模拟生成，建议通过学术数据库检索真实文献)：
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1. **"MYL12B regulates cancer cell motility through cytoskeletal dynamics"**
*Author: Tanaka H, et al.*
摘要：研究利用MYL12B抗体进行免疫印迹和免疫荧光，发现MYL12B通过调控肌动蛋白-肌球蛋白相互作用促进肿瘤细胞迁移，为癌症转移机制提供新见解。
2. **"Proteomic analysis identifies MYL12B as a biomarker in hepatocellular carcinoma"**
*Author: Liu X, et al.*
摘要：通过MYL12B抗体进行组织芯片分析，发现其在肝癌中高表达，并与患者预后不良相关，提示其作为潜在诊断标志物的价值。
3. **"MYL12B-mediated signaling in smooth muscle contraction"**
*Author: Smith J, et al.*
摘要：利用MYL12B抗体阻断实验，揭示其在平滑肌收缩中的关键作用，涉及RhoA/ROCK通路调控，为心血管疾病治疗提供靶点。
4. **"CRISPR screening reveals MYL12B's role in cell division fidelity"**
*Author: Chen R, et al.*
摘要：结合MYL12B抗体敲低实验，发现其缺失导致有丝分裂异常，强调其在维持基因组稳定性中的功能。
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建议通过 **PubMed/Google Scholar** 检索关键词“MYL12B antibody”或“MYL12B function”获取真实文献。
The MYL12B antibody targets the MYL12B protein, also known as myosin regulatory light chain 12B (MLC-2B), a key component of non-muscle myosin II (NMII) complexes. Encoded by the MYL12B gene, this protein regulates actomyosin contractility, influencing critical cellular processes such as cytokinesis, cell migration, and cytoskeletal organization. MYL12B is one of three regulatory light chain isoforms (MYL12A, MYL12B, and MYL9) that modulate myosin II ATPase activity in response to phosphorylation, particularly by Rho-associated kinases (ROCK) and myosin light chain kinase (MLCK).
Antibodies against MYL12B are widely used in cell biology and cancer research to study mechanisms of cell motility, metastasis, and mechanical signaling. They help identify phosphorylation states (e.g., at Ser19/Thr18) to assess NMII activation during stress fiber formation or epithelial-mesenchymal transition (EMT). MYL12B overexpression or dysregulation has been linked to tumor progression, making it a potential biomarker in certain cancers.
These antibodies are validated for techniques like Western blotting, immunofluorescence, and immunoprecipitation. Specificity is critical due to high homology among myosin light chain isoforms. Commercial MYL12B antibodies are typically raised in rabbits or mice, with validation in knockdown or knockout models to confirm target selectivity. Their applications extend to studying neurological disorders, cardiovascular diseases, and developmental biology, underscoring MYL12B's role in cellular mechanics and disease pathways.
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