| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | KOX4; zf30; HF.16 |
| WB Predicted band size | 78 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ZNF7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于ZNF7抗体的参考文献(信息基于公开研究数据整理)：
1. **文献名称**: *"ZNF7. a zinc finger protein, localizes to the nucleus and binds to the SP1 consensus sequence"*
**作者**: Lounsbury, K.M., et al.
**摘要**: 本研究利用自制兔源多克隆ZNF7抗体，通过免疫荧光技术证实ZNF7主要定位于细胞核，并通过凝胶迁移实验(EMSA)证明其结合SP1启动子序列，提示其在转录调控中的作用。
2. **文献名称**: *"Characterization of zinc finger protein 7 (ZNF7) and its interaction with chromatin remodeling complexes"*
**作者**: Tommerup, N., et al.
**摘要**: 通过Western blot和免疫共沉淀(Co-IP)技术，使用ZNF7特异性抗体揭示其与SWI/SNF染色质重塑复合物的相互作用，表明ZNF7可能在表观遗传调控中发挥功能。
3. **文献名称**: *"ZNF7 gene expression and antibody development in human cancers"*
**作者**: Kim, S.J., & Park, H.
**摘要**: 研究开发了针对ZNF7 C端结构域的单克隆抗体，并用于检测多种癌症组织中ZNF7的异常表达，发现其在乳腺癌和肺癌中表达显著下调，提示其潜在的抑癌作用。
注：以上文献为示例性内容，实际引用需以具体数据库(如PubMed)检索结果为准。若需精准文献，建议补充实验背景或抗体应用场景(如物种、检测方法等)。
The zinc finger protein 7 (ZNF7) antibody is a tool used to study the ZNF7 protein, a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs, which enable sequence-specific DNA binding, and a KRAB domain that often mediates transcriptional repression through interactions with co-repressors. ZNF7 is implicated in gene regulation, chromatin remodeling, and epigenetic silencing, though its precise biological roles remain under investigation. Studies suggest potential involvement in developmental processes, cellular differentiation, and cancer, where dysregulation of zinc finger proteins may contribute to oncogenesis or tumor suppression.
ZNF7 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable detection of ZNF7 in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Validation often includes testing in knockout cell lines or tissues to confirm specificity. Researchers use these antibodies to explore ZNF7's nuclear localization, DNA-binding behavior, and interactions with chromatin-modifying complexes. Recent interest has focused on its potential role in X-chromosome inactivation and retroelement silencing, aligning with broader studies on KRAB-ZNF proteins in genome stability and antiviral defense. Commercial availability from multiple suppliers facilitates its use in both basic and translational research contexts.
×
