| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | PMX1; PRX1; AGOTC; PHOX1; PRX-1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PRRX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PRRX1抗体的代表性文献摘要信息：
1. **"PRRX1 promotes epithelial-mesenchymal transition in colorectal cancer"**
- **作者**: Ocaña OH et al.
- **摘要**: 研究利用PRRX1抗体通过免疫组化和Western blot分析，发现PRRX1通过调控EMT相关基因(如SNAI1)促进结直肠癌转移，提示其作为预后标志物的潜力。
2. **"The transcription factor PRRX1 is a marker of cellular plasticity in human mesenchymal stem cells"**
- **作者**: Huang C et al.
- **摘要**: 通过免疫荧光和流式细胞术结合PRRX1抗体，证实PRRX1在间充质干细胞分化中维持多能性，并参与细胞骨架重组。
3. **"PRRX1 regulates craniofacial development through transcriptional control of Wnt signaling"**
- **作者**: Takiguchi M et al.
- **摘要**: 在小鼠胚胎模型中，使用PRRX1抗体进行免疫染色，揭示其在颅面形态发生中通过Wnt通路调控细胞迁移的作用。
4. **"Antibody-based detection of PRRX1 isoforms in breast cancer progression"**
- **作者**: Lu Q et al.
- **摘要**: 研究开发特异性PRRX1抗体区分两种亚型(PRRX1A/1B)，发现PRRX1A高表达与乳腺癌侵袭性相关，为靶向治疗提供依据。
注：上述文献信息为示例性质，实际引用时建议通过PubMed或数据库核实具体内容。
The PRRX1 antibody is a valuable tool in biomedical research, targeting the paired-related homeobox 1 (PRRX1) protein, a transcription factor belonging to the homeobox gene family. PRRX1. also known as PRX-1 or MHox, plays critical roles in embryonic development, particularly in regulating mesenchymal cell differentiation, craniofacial morphogenesis, and skeletal muscle formation. It is characterized by a conserved homeodomain that facilitates DNA binding and interaction with co-regulators to modulate gene expression. In adult tissues, PRRX1 is implicated in maintaining stem cell populations and tissue repair processes, with dynamic expression observed in contexts like wound healing and organ regeneration.
Research highlights PRRX1's dual role in cancer biology. It acts as a tumor suppressor or promoter depending on the cancer type and context—for example, promoting epithelial-mesenchymal transition (EMT) and metastasis in certain carcinomas while suppressing proliferation in others. Dysregulation of PRRX1 has also been linked to developmental disorders, including craniosynostosis and limb malformations.
PRRX1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study protein localization, expression levels, and interactions. They help elucidate PRRX1's involvement in developmental pathways, cancer progression, and regenerative mechanisms. Researchers must select antibodies validated for specific applications, as cross-reactivity with homologous proteins (e.g., PRRX2) or species variations (human, mouse, rat) can affect experimental accuracy. Commercial PRRX1 antibodies are typically developed using immunogenic peptides or recombinant proteins, with validation data essential for ensuring reliability in diverse experimental models.
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