| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZNF523; Zfp523; D6S229E |
| WB Predicted band size | 62 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human ZNF76 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZNF76抗体的3篇模拟参考文献示例(请注意，部分文献信息为虚构，实际引用时需核实)：
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1. **文献名称**：*ZNF76 regulates p53-mediated transcription in cancer cells*
**作者**：Smith J, et al.
**摘要**：本研究利用特异性ZNF76抗体进行免疫沉淀和染色质免疫共沉淀(ChIP)，揭示了ZNF76与肿瘤抑制蛋白p53的相互作用机制。通过抑制ZNF76表达，发现其可调控p53靶基因的转录活性，提示其在癌症中的潜在治疗作用。
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2. **文献名称**：*Characterization of a novel monoclonal antibody against human ZNF76*
**作者**：Lee H, et al.
**摘要**：作者开发了一种高特异性单克隆ZNF76抗体，并通过Western blot和免疫荧光验证了其在多种细胞系中的适用性。该抗体成功用于检测ZNF76在细胞核内的定位，并发现其表达水平在分化过程中动态变化。
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3. **文献名称**：*ZNF76 interacts with chromatin remodelers to modulate neural development*
**作者**：Garcia R, et al.
**摘要**：通过ZNF76抗体的免疫组化分析，研究发现ZNF76在小鼠大脑发育过程中高表达。结合CRISPR敲除技术，证实ZNF76通过与染色质重塑复合物(如NuRD)相互作用，影响神经前体细胞的增殖与分化。
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**注意**：以上文献信息为示例性质，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。若需进一步协助定位具体文献，请提供更多研究背景或关键词。
The ZNF76 antibody is a valuable tool for studying the Zinc Finger Protein 76 (ZNF76), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF76. also known as HUB1. is a nuclear protein encoded in humans by the ZNF76 gene on chromosome 16. It contains an N-terminal KRAB domain, which mediates transcriptional repression, and C-terminal C2H2-type zinc finger motifs for DNA binding. ZNF76 functions as a transcriptional repressor, interacting with heterochromatin protein 1 (HP1) to regulate chromatin organization and gene silencing. It plays roles in cell cycle regulation, differentiation, and development.
The antibody is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to detect ZNF76's expression, localization, and DNA-binding activity. It recognizes endogenous ZNF76 across species, including human, mouse, and rat. Research utilizing this antibody has linked ZNF76 to transcriptional regulation of genes involved in stress response and tumor suppression. Notably, studies suggest its dysregulation may contribute to carcinogenesis, with reduced expression observed in certain cancers. Commercial ZNF76 antibodies are typically developed using immunogens corresponding to specific epitopes within the protein's conserved regions, ensuring specificity for targeted applications in molecular and cellular biology research.
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