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产品描述：人可溶性CD163分子(sCD163)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD163含量。CD163是一种细胞表面糖蛋白，主要表达于单核巨噬细胞系统。其在抗炎、清除血红蛋白-触珠蛋白复合物等过程中发挥重要作用。研究机制上，它可作为炎症反应的调节因子，也成为肿瘤免疫治疗等领域潜在靶点，有望为相关疾病干预提供新思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，灵敏度为0.39 ng/mL。适用于微量样本中sCD163的精准定量分析；适用于实验室开展巨噬细胞功能研究、炎症相关疾病模型评估、免疫调控机制探索等科研项目，为免疫学、肿瘤学及代谢疾病研究提供可靠的生物标志物检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C163A_HUMAN ELISA Kit; CD 163 ELISA Kit; CD163 ELISA Kit; CD163 antigen ELISA Kit; CD163 molecule ELISA Kit; Hemoglobin scavenger receptor ELISA Kit; M130 ELISA Kit; M130 antigen precursor ELISA Kit; Macrophage associated antigen ELISA Kit; MM130 ELISA Kit; OTTHUMP00000238617 ELISA Kit; OTTHUMP00000238618 ELISA Kit; OTTHUMP00000238619 ELISA Kit; OTTHUMP00000238620 ELISA Kit; SCARI1 ELISA Kit; Scavenger receptor cysteine rich type 1 protein M130 ELISA Kit; sCD163 ELISA Kit; Soluble CD163 ELISA Kit
缩写：CD163
Uniprot No.：Q86VB7
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD163 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 88 | ||||
| Range % | 83-93 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of human sCD163 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 93-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.4522.5522.5022.368 502.1342.1762.1552.021 251.5391.4971.5181.384 12.50.7280.7390.7340.600 6.250.4120.4240.4180.284 3.120.2290.2390.2340.100 1.560.1940.2010.1980.064 00.1320.1350.134 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CD163 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CD163 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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