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产品描述：犬CC趋化因子5(CCL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL5含量。CCL5是一种趋化因子，在免疫调节、炎症反应等生理和病理过程中发挥重要作用。其研究机制主要围绕与受体结合，引导免疫细胞迁移和活化，参与肿瘤微环境形成、炎症性疾病发展等，在抗肿瘤、抗炎等治疗领域有潜在应用价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.081 ng/mL。该产品适用于实验动物模型研究，如评估犬类炎症性疾病进程、免疫相关病理机制探索或药物干预实验中趋化因子动态变化的定量监测，为兽医学、比较医学及免疫学领域提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
缩写：CCL5
Uniprot No.：Q8HYS0
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.081 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 97-110 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 95-106 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 87-101 | ||||||
| 1:8 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
回收率：
| The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 104 | 96-108 | |||||
| EDTA plasma (n=4) | 99 | 91-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected CV102.6582.6002.6292.510 1.56%51.9852.0162.0011.882 1.10%2.51.3481.3691.3591.240 1.09%1.250.9190.9270.9230.804 0.61%0.6250.5020.5230.5130.394 2.89%0.3130.2940.3140.3040.185 4.65%0.1560.2120.2190.2160.097 2.29.1170.1210.119 2.38% | |||||||
货期：3-5 working days
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