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产品描述：人C-C型趋化因子27(CCL27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCL27含量。CCL27是一个重要靶点。其它是一种趋化因子。在研究机制上，CCL27能与特定受体结合，参与免疫细胞的迁移和归巢过程，在皮肤免疫、炎症反应等生理病理过程中发挥作用，目前正被深入研究以探索疾病治疗新途径。试剂盒检测范围为0.313 ng/mL-20 ng/mL，灵敏度为0.043 ng/mL。适用于基础医学研究中评估皮肤相关疾病的生物标志物水平、炎症反应机制探索以及肿瘤微环境中趋化因子动态变化的定量分析，为免疫调控研究和药物开发提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALP ELISA Kit; C-C motif chemokine 27 ELISA Kit; CC chemokine ILC ELISA Kit; Ccl27 ELISA Kit; CCL27_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 27 ELISA Kit; CTACK ELISA Kit; CTAK ELISA Kit; Cutaneous T cell attracting chemokine ELISA Kit; Cutaneous T-cell-attracting chemokine ELISA Kit; ESkine ELISA Kit; IL 11 R alpha locus chemokine ELISA Kit; IL-11 R-alpha-locus chemokine ELISA Kit; ILC ELISA Kit; PESKY ELISA Kit; SCYA27 ELISA Kit; Skinkine ELISA Kit; Small inducible cytokine A27 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 27 ELISA Kit; Small-inducible cytokine A27 ELISA Kit
缩写：CCL27
Uniprot No.：Q9Y4X3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.313 ng/mL-20 ng/mL
灵敏度：0.043 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCL27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 85 | |||||
| Range % | 81-88 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-101 | ||||||
| 1:4 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
回收率：
| The recovery of human CCL27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 94-100 | |||||
| EDTA plasma (n=4) | 105 | 101-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.7152.7682.7422.589 102.0972.0142.0561.903 51.2761.2341.2551.102 2.50.7520.7680.7600.607 1.250.4810.4980.4900.337 0.6250.3270.3100.3190.166 0.3130.2570.2440.2510.098 00.1560.1500.153 | |||||||
货期：3-5 working days
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