首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CC趋化因子5 (CCL5/D17S136E/SCYA5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCL5含量。CCL5是一个重要靶点。它属于趋化因子家族，在免疫调节、炎症反应等生理病理过程中发挥关键作用。其研究机制主要围绕其与受体结合，介导细胞迁移和活化。对CCL5的研究有助于深入了解疾病发病机制，为相关疾病治疗提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为2.307 pg/mL。适用于体外研究其在不同疾病模型中的表达动态、药物干预对炎症通路的影响，或评估免疫微环境中趋化因子的调控机制；为炎症机制研究、免疫调控实验及抗病毒药物开发等科研领域提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Beta chemokine RANTES ELISA Kit; Beta chemokine RANTES precursor ELISA Kit; C C motif chemokine 5 ELISA Kit; CCL 5 ELISA Kit; CCL5 ELISA Kit; CCL5_HUMAN ELISA Kit; Chemokine (C C motif) ligand 5 ELISA Kit; Chemokine CC Motif Ligand 5 ELISA Kit; D17S136E ELISA Kit; EoCP ELISA Kit; Eosinophil chemotactic cytokine ELISA Kit; MGC17164 ELISA Kit; RANTES(4-68) ELISA Kit; Regulated upon activation normally T expressed and presumably secreted ELISA Kit; SCYA 5 ELISA Kit; SCYA5 ELISA Kit; SIS delta ELISA Kit; SIS-delta ELISA Kit; SISd ELISA Kit; Small inducible cytokine A5 (RANTES) ELISA Kit; Small inducible cytokine A5 ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 5 ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T cell specific protein p288 ELISA Kit; T cell specific protein RANTES ELISA Kit; T cell specific RANTES protein ELISA Kit; T cell-specific protein P228 ELISA Kit; T-cell-specific protein RANTES ELISA Kit; TCP 228 ELISA Kit; TCP228 ELISA Kit
缩写：CCL5
Uniprot No.：P13501
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：2.307 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:200 | Average % | 104 | ||||
| Range % | 96-107 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 86-98 | ||||
| EDTA plasma (n=4) | 99 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.6712.5422.6072.511 5001.8481.7761.8121.716 2501.0261.0071.0170.921 1250.5750.5830.5790.483 62.50.3320.3440.3380.242 31.20.2580.2470.2530.157 15.60.1600.1720.1660.070 00.0980.0940.096 | ||||||
货期：3-5 working days
×
