Rabbit Polyclonal VPS28 Antibody

The VPS28 antibody is a key tool in studying the endosomal sorting complex required for transport (ESCRT) system, which plays a critical role in cellular membrane dynamics. VPS28 is a core component of ESCRT-I, a multiprotein complex involved in sorting ubiquitinated cargo proteins into intralumenal vesicles of multivesicular bodies (MVBs) and facilitating processes like viral budding, cytokinesis, and autophagy. Structurally, VPS28 bridges ESCRT-I to ESCRT-II through interactions with VPS25. enabling sequential recruitment of ESCRT complexes during vesicle formation. Research using VPS28 antibodies focuses on elucidating its role in membrane remodeling, particularly in pathologies such as neurodegenerative diseases, cancer, and viral infections (e.g., HIV-1). These antibodies are commonly employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, localization, and interaction partners. Studies have linked VPS28 dysfunction to impaired MVB formation, defective lysosomal degradation, and abnormal cell signaling, highlighting its importance in maintaining cellular homeostasis. As part of the evolutionarily conserved ESCRT machinery, VPS28 is also investigated in developmental biology and organelle biogenesis. Commercial VPS28 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes across human, mouse, or rat homologs. Validation includes knockout controls and functional assays to ensure specificity, making them indispensable for exploring ESCRT-mediated processes in health and disease.