| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | WIP1; PP2C-DELTA |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PPM1D |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPM1D抗体的3篇代表性文献摘要概括(示例性质，非真实文献)：
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1. **"PPM1D/Wip1 phosphatase regulates p53-dependent apoptosis in cancer cells"**
*Author: Bulavin DV, et al.*
该研究利用PPM1D特异性抗体，通过Western blot和免疫组化发现PPM1D通过去磷酸化p53抑制其活性，促进肿瘤细胞逃避凋亡，揭示了其在癌症中的关键作用。
2. **"Characterization of Wip1 phosphatase monoclonal antibody for DNA damage studies"**
*Author: Fiscella M, et al.*
文章报道了一种高特异性抗PPM1D单克隆抗体的开发，验证其在流式细胞术和免疫沉淀中的有效性，并证明PPM1D在DNA损伤修复通路中的负反馈调节功能。
3. **"Elevated expression of PPM1D confers poor prognosis in ovarian cancer"**
*Author: Zhang Y, et al.*
通过免疫组化分析临床样本，发现PPM1D在卵巢癌中高表达且与化疗耐药相关，抗体检测结果提示其可作为潜在预后标志物和治疗靶点。
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注：以上内容为模拟摘要，实际文献需通过PubMed或Google Scholar检索关键词(如PPM1D antibody、Wip1 phosphatase)获取。
**Background of PPM1D Antibody**
PPM1D (Protein Phosphatase, Mg²⁺/Mn²⁺-Dependent 1D), also known as Wip1 (Wild-type p53-Induced Phosphatase 1), is a serine/threonine phosphatase belonging to the PP2C family. It plays a critical role in regulating cellular stress responses, particularly in the DNA damage response (DDR) pathway. PPM1D is transcriptionally induced by p53 following genotoxic stress and functions as a negative feedback regulator by dephosphorylating key DDR proteins, including p53. ATM, CHK1. and CHK2. thereby attenuating cell cycle arrest and apoptosis.
Overexpression or gain-of-function mutations in *PPM1D* are linked to various cancers, such as breast cancer, ovarian cancer, and neuroblastoma, due to its oncogenic potential in suppressing tumor suppressor pathways. Conversely, *PPM1D* deletions or truncations are associated with developmental disorders and immunodeficiency.
PPM1D antibodies are essential tools for studying its expression, localization, and functional roles in cancer biology and stress signaling. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect PPM1D protein levels in tissues or cell lines. These antibodies also aid in exploring PPM1D's interaction networks and evaluating its potential as a therapeutic target, particularly in cancers with *PPM1D* amplifications or mutations. Specific monoclonal and polyclonal antibodies targeting distinct epitopes or post-translational modifications enable precise mechanistic insights into PPM1D-driven pathologies.
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