WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/25-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
Aliases | HASH; SRF1; SRF-1 |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | Fusion protein of human SPATA6 |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于SPATA6抗体的3篇假设性参考文献,基于典型研究场景模拟:
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1. **标题**: *SPATA6 regulates ciliogenesis through microtubule stabilization in murine embryonic development*
**作者**: Chen L, et al.
**摘要**: 本研究利用SPATA6特异性抗体进行免疫沉淀和免疫荧光实验,发现SPATA6通过稳定中心体微管结构调控纤毛形成,其缺失导致小鼠胚胎发育异常。抗体验证表明SPATA6在纤毛基部分布显著。
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2. **标题**: *A novel role of SPATA6 in mitochondrial function and oxidative stress response*
**作者**: Gupta R, et al.
**摘要**: 通过Western blot和共聚焦显微镜(使用兔源SPATA6多克隆抗体),证实SPATA6与线体内膜蛋白相互作用,调控细胞氧化应激反应。抗体特异性经siRNA敲低实验验证。
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3. **标题**: *SPATA6 antibody-based profiling reveals altered expression in human asthenozoospermia*
**作者**: Wang Y, et al.
**摘要**: 采用SPATA6单克隆抗体对弱精症患者睾丸组织进行免疫组化分析,发现SPATA6表达水平与精子活力正相关,提示其作为男性不育潜在生物标志物的价值。
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*注:以上文献为示例性质,实际研究中需查询真实数据库获取准确信息。*
**Background of SPATA6 Antibody**
The SPATA6 (spermatogenesis-associated protein 6) antibody is a tool used to study the SPATA6 protein, encoded by the *SPATA6* gene, which is primarily associated with reproductive biology, particularly spermatogenesis. SPATA6 is expressed predominantly in the testis and plays a critical role in sperm development, including acrosome formation and sperm head shaping. It interacts with other proteins, such as HOOK1 and GOPC, to regulate vesicular trafficking during spermatogenesis. Dysregulation of SPATA6 has been linked to male infertility due to its essential role in sperm maturation and structural integrity.
Beyond reproductive health, SPATA6 has been implicated in certain cancers, with studies suggesting its overexpression in tumor tissues. The SPATA6 antibody is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interactions in cellular and tissue samples. Research employing this antibody contributes to understanding molecular mechanisms underlying infertility and oncogenesis. Commercial SPATA6 antibodies are typically developed using recombinant protein fragments or specific epitopes, validated for specificity across human and model organisms. Ongoing studies aim to explore its diagnostic or therapeutic potential in reproductive disorders and cancer.
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