| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MI; WS2; CMM8; WS2A; COMMAD; bHLHe32 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MITF |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MITF抗体的3篇参考文献示例(注：文献信息为模拟生成，仅作参考)：
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1. **文献名称**：*MITF in melanoma: Mechanisms behind its function as a molecular oncogene*
**作者**：Levy, C., et al.
**摘要**：研究探讨了MITF在黑色素瘤中的调控机制，验证了多种MITF抗体的特异性，并发现其通过调控下游靶基因(如TYR、MLANA)影响肿瘤增殖和转移。抗体应用于免疫组化(IHC)和Western blot分析。
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2. **文献名称**：*Diagnostic utility of MITF antibody in differentiating melanocytic tumors*
**作者**：King, R., et al.
**摘要**：评估MITF抗体作为黑色素细胞肿瘤诊断标志物的价值，对比不同克隆抗体(如C5、D5)的敏感性和特异性，证实其在区分良性痣与恶性黑色素瘤中的临床应用潜力。
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3. **文献名称**：*MITF isoforms and their roles in melanoma progression*
**作者**：Garraway, L.A., et al.
**摘要**：通过CRISPR和小分子抑制剂实验，分析MITF不同亚型的生物学功能，并利用特异性抗体(如克隆号HPA003235)验证其在细胞核内的定位及与BRAF突变黑色素瘤的关联性。
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(如需真实文献，建议通过PubMed或Google Scholar检索关键词“MITF antibody diagnostic”或“MITF melanoma IHC”获取近年研究。)
The Microphthalmia-associated transcription factor (MITF) is a critical regulatory protein belonging to the basic helix-loop-helix leucine zipper (bHLH-Zip) family. It plays a central role in the development, survival, and function of various cell types, including melanocytes, retinal pigment epithelium, mast cells, and osteoclasts. MITF regulates the expression of genes involved in melanin synthesis (e.g., *TYR*, *TYRP1*, *DCT*), cell cycle progression, and apoptosis, making it indispensable for melanocyte differentiation and pigment production.
In pathology, MITF is closely linked to melanoma, where its dysregulation—through amplification, mutation, or aberrant signaling—often drives tumor progression and metastasis. Additionally, MITF mutations are associated with rare genetic disorders like Waardenburg syndrome type 2A and Tietz syndrome, characterized by pigmentation defects and hearing loss.
MITF-specific antibodies are vital tools in research and diagnostics. They enable the detection of MITF expression levels and subcellular localization via techniques like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Clinically, MITF antibodies aid in diagnosing melanocytic tumors, distinguishing melanoma from other malignancies (e.g., sarcoma vs. carcinoma), and assessing metastatic potential. Their specificity also supports studies exploring MITF's interplay with pathways like MAPK/ERK and its role in drug resistance, offering insights for targeted therapies.
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