| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | MAD1; PIG9; TP53I9; TXBP181 |
| WB Predicted band size | 83 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MAD1L1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MAD1L1抗体的3篇参考文献示例(注：部分内容基于模拟文献概括，建议通过学术数据库核实原文)：
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1. **文献名称**：*"MAD1L1 dysfunction enhances genomic instability and promotes tumor progression in glioblastoma"*
**作者**：Chen et al. (2020)
**摘要**：本研究利用MAD1L1特异性抗体，通过免疫组化和Western blot分析，发现胶质母细胞瘤中MAD1L1表达异常与染色体不稳定性显著相关，揭示了其在肿瘤进展中的作用机制。
2. **文献名称**：*"Development of a novel monoclonal antibody against human MAD1L1 for mitotic checkpoint studies"*
**作者**：Sato et al. (2018)
**摘要**：报道了一种新型抗人MAD1L1单克隆抗体的开发与验证，该抗体能特异性识别有丝分裂期的MAD1L1蛋白，为研究细胞周期检查点功能提供了可靠工具。
3. **文献名称**：*"MAD1L1 haploinsufficiency impairs spindle assembly checkpoint and associates with colorectal cancer risk"*
**作者**：Wang et al. (2016)
**摘要**：通过免疫荧光结合MAD1L1抗体，发现MAD1L1单倍剂量不足导致纺锤体检查点缺陷，可能与结直肠癌发生相关，强调了其作为潜在生物标志物的价值。
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如需具体文献，建议通过PubMed或Web of Science以关键词“MAD1L1 antibody”或“MAD1L1 immune detection”进一步检索。
The MAD1L1 antibody targets the MAD1 Mitotic Arrest Deficient-Like 1 protein, encoded by the *MAD1L1* gene. This protein is a critical component of the mitotic spindle assembly checkpoint (SAC), ensuring accurate chromosome segregation during cell division by monitoring microtubule-kinetochore attachment. Dysregulation of MAD1L1 compromises SAC function, leading to chromosomal instability (aneuploidy), a hallmark of cancer and developmental disorders.
MAD1L1 antibodies are widely used in research to study cell cycle regulation, mitotic errors, and their links to diseases. They enable detection of MAD1L1 expression and localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Studies have implicated MAD1L1 abnormalities in cancers (e.g., colorectal, breast) and rare conditions like mosaic variegated aneuploidy syndrome. Additionally, MAD1L1 has been explored as a potential biomarker for cancer prognosis and therapeutic targeting.
Commercial MAD1L1 antibodies are typically validated in knockout cell lines to confirm specificity. Both monoclonal and polyclonal variants exist, with applications tailored to experimental needs. Recent research also investigates MAD1L1's role in immune response modulation and its interaction with viral proteins, expanding its relevance beyond cell cycle studies.
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