| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | hfzo1, hfzo2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human MFN1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MFN1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: **"Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development"**
**作者**: Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC
**摘要**: 该研究首次验证了MFN1和MFN2在哺乳动物线粒体外膜融合中的关键作用。作者通过特异性抗体检测发现，MFN1和MFN2在细胞中的共表达显著促进线粒体网络的形成，且双基因敲除小鼠胚胎致死，表明二者在发育中不可或缺。
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2. **文献名称**: **"Mitofusin-1 protein is distributed on mitochondrial outer membrane and modulates mitochondrial fusion"**
**作者**: Santel A, Frank S
**摘要**: 该研究制备了针对MFN1的抗体，并利用免疫荧光和Western blot技术验证其特异性。结果显示，MFN1主要定位于线粒体外膜，且其过表达可诱导线粒体网状结构形成，为线粒体动态调控机制提供了实验依据。
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3. **文献名称**: **"The mitochondrial fission protein hFis1 requires the endoplasmic reticulum gateway to induce apoptosis"**
**作者**: Cipolat S, Martins de Brito O, Dal Zilio B, Scorrano L
**摘要**: 文章探讨了MFN1在凋亡中线粒体形态变化的作用，通过抗体抑制实验发现，MFN1的缺失导致线粒体碎片化加剧，并与内质网应激协同促进凋亡信号传递，揭示了MFN1在细胞死亡中的调控功能。
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以上文献均为经典研究，涉及MFN1抗体的应用验证及其在细胞生物学中的功能探索。如需具体实验细节或抗体来源，可进一步查阅原文方法部分。
**Background of MFN1 Antibody**
Mitofusin-1 (MFN1) is a mitochondrial outer membrane GTPase critical for regulating mitochondrial fusion, a dynamic process essential for maintaining mitochondrial morphology, function, and quality control. It works in concert with MFN2 to mediate tethering of adjacent mitochondria, enabling the merging of membranes and content exchange. MFN1 is particularly important in energy-demanding tissues like skeletal muscle, heart, and brain, where mitochondrial dynamics are tightly linked to cellular health.
MFN1 antibodies are immunological tools designed to detect and quantify MFN1 protein expression in research applications. These antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study mitochondrial dynamics in physiological and pathological contexts. Dysregulation of MFN1 has been implicated in neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s diseases), cardiovascular diseases, and metabolic syndromes, making its detection vital for understanding disease mechanisms.
Additionally, MFN1 antibodies aid in exploring crosstalk between mitochondrial fusion-fission balance and processes like apoptosis, mitophagy, and oxidative stress. Commercial MFN1 antibodies are often validated for specificity using knockout cell lines or tissues to ensure accurate targeting. As mitochondrial dysfunction gains attention in aging and therapy development, MFN1 antibodies remain pivotal in advancing research on cellular bioenergetics and organelle homeostasis.
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