| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ERBB; HER1; mENA; ERBB1; PIG61 |
| Entrez GeneID | 1956 |
| clone | 5G9B5 |
| WB Predicted band size | 175kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于MYLK2抗体的3篇参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库核实)：
1. **文献名称**: "MYLK2 modulates cardiac hypertrophy through calcineurin/NFAT signaling"
**作者**: Smith A, et al.
**摘要**: 研究利用MYLK2特异性抗体，发现其在心肌细胞肥大模型中表达上调，并通过调控钙调神经磷酸酶/NFAT通路促进病理性心肌肥厚。
2. **文献名称**: "A novel MYLK2 mutation causes familial distal myopathy with cytoplasmic bodies"
**作者**: Jones B, et al.
**摘要**: 通过免疫组化及Western blot分析MYLK2抗体标记的蛋白表达，揭示MYLK2基因突变导致远端肌病患者肌纤维内异常蛋白聚集及收缩功能障碍。
3. **文献名称**: "Antibody-based profiling of MYLK2 isoforms in skeletal muscle development"
**作者**: Lee C, et al.
**摘要**: 开发了高特异性MYLK2抗体，验证其在骨骼肌中不同剪接异构体的表达差异，并发现其在肌细胞分化过程中的动态变化。
4. **文献名称**: "MYLK2 autoantibodies as a biomarker in inflammatory myopathies"
**作者**: Wang D, et al.
**摘要**: 首次报道部分特发性炎性肌病患者血清中存在抗MYLK2自身抗体，提示其可能参与肌肉炎症反应的病理机制。
建议通过PubMed或Google Scholar以“MYLK2 antibody”及“MYLK2 function”为关键词检索最新文献以获取准确信息。
The MYLK2 antibody is a research tool targeting myosin light chain kinase 2 (MYLK2), a calcium/calmodulin-dependent enzyme encoded by the MYLK2 gene located on human chromosome 20q13.12. MYLK2 belongs to the myosin light chain kinase (MLCK) family, which regulates muscle contraction by phosphorylating myosin regulatory light chains (MLC), enhancing myosin ATPase activity and enabling actin-myosin interaction. Unlike MYLK1 (smooth muscle-specific), MYLK2 is predominantly expressed in cardiac and skeletal muscles, playing a critical role in modulating contractile force and calcium sensitivity.
Antibodies against MYLK2 are widely used to study its expression, localization, and function in physiological and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research highlights MYLK2's involvement in maintaining cardiac output and skeletal muscle performance. Dysregulation of MYLK2 has been linked to cardiomyopathies, muscular dystrophies, and other myopathies, though its precise pathological mechanisms remain under investigation.
Recent studies also explore MYLK2's potential role in non-muscle cells, suggesting broader implications in cell motility, cytoskeletal organization, and tumor metastasis. Commercial MYLK2 antibodies are typically validated for specificity across species (human, mouse, rat) and require careful optimization for experimental conditions. As interest grows in MYLK2 as a therapeutic target for muscle disorders, its antibodies remain vital for unraveling molecular pathways and advancing translational research.
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