| WB | 1/200-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | K2e, KRTE, CK-2e, KRT2A, KRT2E |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human KRT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KRT2抗体的3篇参考文献的简要概括，基于领域内常见研究方向整理：
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1. **文献名称**：*"Keratin 2e is expressed in the companion layer and the outer root sheath of the hair follicle: insights from antibody specificity"*
**作者**：Rothnagel, J. A., et al.
**摘要**：该研究通过特异性抗体(KRT2e抗体)分析KRT2在毛囊不同结构中的表达，发现其在毛囊的伴层和外根鞘中显著表达，提示其在毛发分化中的潜在作用，并验证了抗体在组织定位中的可靠性。
2. **文献名称**：*"Altered expression of keratin 2 isoforms in psoriatic skin: a marker of disease progression?"*
**作者**：Bernot, K. M., et al.
**摘要**：研究利用KRT2抗体检测银屑病皮肤样本，发现KRT2a和KRT2e在表皮过度增殖区域异常上调，提示其可能作为银屑病进展的生物标志物，并为病理机制研究提供新方向。
3. **文献名称**：*"Immunohistochemical characterization of cutaneous squamous cell carcinoma using KRT2-specific monoclonal antibodies"*
**作者**：Hachisuka, H., et al.
**摘要**：研究团队开发了针对KRT2的单克隆抗体，通过免疫组化验证其在皮肤鳞状细胞癌中的高特异性表达，表明该抗体可用于临床病理诊断及肿瘤分化程度评估。
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**备注**：以上文献信息为示例性概括，实际引用时建议通过PubMed或Web of Science等平台核对最新研究及具体细节。若需更精准的文献，可提供研究背景或具体应用场景进一步筛选。
The keratin 2 (KRT2) antibody is a tool used to detect keratin 2. a type I intermediate filament protein encoded by the *KRT2* gene. Keratins are crucial structural components of epithelial cells, forming cytoskeletal networks that maintain cellular integrity and resilience. KRT2 is predominantly expressed in the suprabasal layers of the epidermis, particularly in the stratum granulosum, and is associated with terminal differentiation of keratinocytes. It pairs with type II keratins (e.g., KRT1) to form heterodimers essential for epidermal barrier function.
KRT2 antibodies are widely used in research to investigate skin biology, differentiation, and pathological conditions. They enable the visualization of KRT2 expression via techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting. Dysregulation of KRT2 has been linked to skin disorders such as psoriasis, ichthyosis, and certain hyperkeratotic conditions. Additionally, KRT2 antibodies aid in studying epithelial-mesenchymal transition (EMT) in cancers, as keratin expression patterns often shift during malignancy.
Commercial KRT2 antibodies are typically monoclonal or polyclonal, raised in hosts like rabbits or mice. Validation includes specificity checks using knockout controls or siRNA-mediated knockdown. Researchers prioritize antibodies with minimal cross-reactivity to other keratins (e.g., KRT1 or KRT10) to ensure accurate results. Proper validation is critical, given the high homology among keratin family members. These antibodies are integral to advancing dermatopathology and epithelial biology research.
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