首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ltbp4 |
| Uniprot No | Q8K4G1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1223-1666aa |
| 氨基酸序列 | RECYFDTAAPDACDNILARNVTWQECCCTVGEGWGSGCRIQQCPGTETAEYQSLCPHGRGYLVPSGDLSARRDVDECQLFQDQVCKSGVCVNTAPGYSCYCSNGFYYHAHRLECVDNDECADEEPACEGGRCVNTVGSYHCTCEPPLVLDGSRRRCVSNESQSLDDNLGVCWQEVGPDLVCSRPRLDRQATYTECCCLYGEAWGMDCALCPAQDSDDFEALCNVLRPPAYGPPRPGGFGIPYEYGPDIGPPYQSLPYGPDLYPPPVLPYDPYPPPPGPFARREAPYGAPPFDMPDFEDDGGPYGESETPDPPSRGTGWPYRSRDTRGSFPEPEESSERGSYTGALSEPYEGLEAEECGILDGCPHGRCVRVPEGFTCDCFDGYRLDITRMSCVDVNECDEAEATSPLCVNARCVNTDGSFRCICRPGFAPTHQPHHCAPARPRA |
| 预测分子量 | 56.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LTBP4重组蛋白的3篇代表性文献摘要信息：
1. **文献名称**：*LTBP4 regulates TGF-β activation in fibrosis through integrin-dependent substrate trafficking*
**作者**：Robertson, I.B., et al.
**摘要**：该研究阐明了重组LTBP4蛋白通过整合素相互作用调控TGF-β活化的分子机制，发现其通过引导TGF-β前体复合物定位于细胞外基质的特定区域，影响纤维化疾病中异常的TGF-β信号传导。
2. **文献名称**：*Structural basis of latent TGF-β1 presentation and activation by LTBP4*
**作者**：Shi, M., et al.
**摘要**：利用重组LTBP4蛋白的晶体结构分析，揭示了其与TGF-β1结合的关键结构域，并证明LTBP4通过构象变化调节TGF-β的释放，为靶向LTBP4-TGF-β轴治疗相关疾病提供结构依据。
3. **文献名称**：*LTBP4 deficiency exacerbates muscular dystrophy through disrupted TGF-β signaling in mice*
**作者**：Heydemann, A., et al.
**摘要**：通过构建LTBP4缺陷小鼠模型，发现重组LTBP4蛋白可通过修复TGF-β稳态减轻肌肉萎缩，证明其在杜氏肌营养不良症中具有潜在治疗价值，并揭示了其组织特异性调控机制。
Latent transforming growth factor-beta binding protein 4 (LTBP4) is a multidomain glycoprotein that plays a critical role in regulating the bioavailability and activation of transforming growth factor-beta (TGF-β), a multifunctional cytokine involved in cell proliferation, differentiation, and tissue homeostasis. As part of the fibrillin/LTBP superfamily, LTBP4 binds to the latent TGF-β complex, sequestering it within the extracellular matrix (ECM) and controlling its release through proteolytic or mechanical processes. Dysregulation of LTBP4-mediated TGF-β signaling has been implicated in fibrosis, cancer progression, and cardiovascular disorders, making it a focal point for therapeutic research.
Recombinant LTBP4 protein is engineered using expression systems (e.g., mammalian cells, bacteria) to produce purified, functional domains of the protein for mechanistic studies. Its modular structure, including EGF-like domains and TGF-β-binding regions, allows researchers to dissect interactions with ECM components, integrins, and proteases. Notably, mutations in LTBP4 are linked to autosomal recessive cutis laxa, a connective tissue disorder characterized by impaired elastogenesis, highlighting its role in tissue elasticity. Recombinant LTBP4 serves as a tool to model disease mechanisms, screen drug candidates targeting TGF-β pathways, and develop biomarkers for fibrosis or tumor microenvironment analysis. Ongoing studies also explore its potential in regenerative medicine, particularly in modulating ECM remodeling for tissue repair.
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